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Acetyl-Histone H3 (Lys18) Recombinant antibody, PBS Only

Acetyl-Histone H3 (Lys18) Uni-rAbTM Recombinant Antibody for WB, Dot Blot, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Dot Blot, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2E3

Cat no : 82832-1-PBS

Print datasheet

Synonyms

HIST1H3A, 2E3, Histone-H3, HistoneH3, Histone H3

验证数据展示

  • Dot Blot experiment of peptide using 82832-1-RR (same clone as 82832-1-PBS)

    Dot Blot experiment of peptide using 82832-1-RR (same clone as 82832-1-PBS)

    Dot blot analysis was used to confirm the specificity of 82832-1-RR Acetyl-Histone H3 (Lys18) antibody. Acetylated peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row. Column 1: H3AK18Ac. Column 2: Unmodified H3AK18. Column 3: H3AK36Ac. Column 4: Unmodified H3AK36. Column 5: H3AK23Ac. Column 6: Unmodified H3AK23. Column 7: H3AK122Ac. Column 8:Unmodified H3AK122. Column 9: H3AK27Ac. Column 10: Unmodified H3AK27. Column 11: H3AK14Ac. Column 12: Unmodified H3AK14. Column 13: H3AK56Ac. Column 14:Unmodified H3AK56. Column 15:H3AK9Ac. Column 16:Unmodified H3AK9. Column 17:H3AK4Ac. Column 18:Unmodified H3AK4. Column 19:Blank(PBS). This data was developed using the same antibody clone with 82832-1-PBS in a different storage buffer formulation.

  • WB analysis of NIH/3T3 using 82832-1-RR (same clone as 82832-1-PBS)

    WB analysis of NIH/3T3 using 82832-1-RR (same clone as 82832-1-PBS)

    Trichostatin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 82832-1-RR (Acetyl-Histone H3 (Lys18) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82832-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy Phospho-Histone H3 (Ser10) Ready-To-Use IHC Kit. Phospho-Histone H3 (Ser10) primary antibody included.

产品信息

82832-1-PBS targets Acetyl-Histone H3 (Lys18) in WB, Dot Blot, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, Dot Blot, Indirect ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name histone cluster 1, H3a
Synonyms HIST1H3A, 2E3, Histone-H3, HistoneH3, Histone H3
GenBank Accession NumberBC066245
Gene Symbol HIST1H3A
Gene ID (NCBI) 8350
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP68431
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C