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CoraLite® Plus 488-conjugated Acetyl-Histone H4 (Lys12) Recombinant antibody
Acetyl-Histone H4 (Lys12) Uni-rAbTM Recombinant Antibody for IF/ICC
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1D18
验证数据展示
经过测试的应用
| Positive IF/ICC detected in | Caco-2 cells |
For other applications, we recommend the unconjugated version of this antibody, 83095-1-PBS
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-83095 targets Acetyl-Histone H4 (Lys12) in IF/ICC applications and shows reactivity with human, mouse samples.
| Tested Applications | IF/ICC Application Description |
| Tested Reactivity | human, mouse |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | histone cluster 1, H4a |
| Synonyms | HIST1H4A, 1D18, Histone-H4, histoneH4, Histone H4 |
| Observed Molecular Weight | 12 kDa |
| GenBank Accession Number | BC069654 |
| Gene Symbol | HIST1H4A |
| Gene ID (NCBI) | 8359 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P62805 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Histone H4 is a 103 amino acid protein, which belongs to the histone H4 family. Histone H4 localizes in the nucleus and is a core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Acetylation of histone H4 is necessary for chromatin decompaction during DNA replication.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Acetyl-Histone H4 (Lys12) antibody CL488-83095 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |