• Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Acetyl-Histone H4 (Lys12) Recombinant antibody

Acetyl-Histone H4 (Lys12) Uni-rAbTM Recombinant Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1D18

Cat no : CL488-83095

Print datasheet

Synonyms

HIST1H4A, 1D18, Histone-H4, histoneH4, Histone H4



经过测试的应用

Positive IF/ICC detected inCaco-2 cells
For other applications, we recommend the unconjugated version of this antibody, 83095-1-PBS

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-83095 targets Acetyl-Histone H4 (Lys12) in IF/ICC applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name histone cluster 1, H4a
Synonyms HIST1H4A, 1D18, Histone-H4, histoneH4, Histone H4
Observed Molecular Weight12 kDa
GenBank Accession NumberBC069654
Gene Symbol HIST1H4A
Gene ID (NCBI) 8359
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP62805
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Histone H4 is a 103 amino acid protein, which belongs to the histone H4 family. Histone H4 localizes in the nucleus and is a core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Acetylation of histone H4 is necessary for chromatin decompaction during DNA replication.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Acetyl-Histone H4 (Lys12) antibody CL488-83095Download protocol
Standard Protocols
Click here to view our Standard Protocols