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验证数据展示

  • IF Staining of 3T3-L1 using CL488-66239

    IF Staining of 3T3-L1 using CL488-66239

    Immunofluorescent analysis of (-20°C Methanol) fixed 3T3-L1 cells using CoraLite®488 Adiponectin antibody (CL488-66239, Clone: 5D8A7 ) at dilution of 1:200.

CoraLite®488-conjugated Adiponectin Monoclonal antibody

Adiponectin Monoclonal Antibody for IF/ICC
Cat No. CL488-66239

Print datasheet

CloneNo. 5D8A7

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

IF/ICC

Synonyms

ACDC, ACRP30, adiponectin, ADIPOQ, ADIPQTL1, ADPN, APM 1, APM1, GBP28, Gelatin binding protein

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected in3T3-L1 cells
Planning an IHC experiment? We recommend our IHCeasy Adiponectin Ready-To-Use IHC Kit. Adiponectin primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66239-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66239 targets Adiponectin in IF/ICC applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse
Immunogen Adiponectin fusion protein Ag17383 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name adiponectin, C1Q and collagen domain containing
Synonyms ACDC, ACRP30, adiponectin, ADIPOQ, ADIPQTL1, ADPN, APM 1, APM1, GBP28, Gelatin binding protein
Calculated Molecular Weight 244 aa, 26 kDa
Observed Molecular Weight 29 kDa
GenBank Accession NumberBC096308
Gene Symbol Adiponectin
Gene ID (NCBI) 9370
RRIDAB_2923753
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths491 nm / 516 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ15848
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Adiponectin (AdipoQ), an adipocyte-derived hormone, is one of the most abundant adipokines in the blood circulation. Adiponectin modulates a number of metabolic processes, including improving insulin sensitivity and anti-inflammatory activity. The role of AdipoQ in reproduction is not yet fully understood, but the expression of AdipoQ in reproductive tissues has been observed in various animals and humans, including chicken testis, bovine ovary, and human placenta. Adiponectin exerts its effects by activating a range of different signaling molecules via binding to two transmembrane AdipoQ receptors, AdipoR1 and AdipoR2. AdipoR1 is expressed primarily in the skeletal muscle, whereas AdipoR2 is predominantly expressed in the liver. AdipoQ May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors.

实验方案

Product Specific Protocols
IF protocol for CL488 Adiponectin antibody CL488-66239Download protocol
Standard Protocols
Click here to view our Standard Protocols