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验证数据展示

  • IF Staining of 3T3-L1 using CL488-66239

    IF Staining of 3T3-L1 using CL488-66239

    Immunofluorescent analysis of (-20°C Methanol) fixed 3T3-L1 cells using CoraLite®488 Adiponectin antibody (CL488-66239, Clone: 5D8A7 ) at dilution of 1:200.

CoraLite®488-conjugated Adiponectin Monoclonal antibody

Adiponectin Monoclonal Antibody for IF/ICC
Cat No. CL488-66239

产品说明书

CloneNo. 5D8A7

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

IF/ICC

别名

ACDC, ACRP30, adiponectin, ADIPOQ, ADIPQTL1, ADPN, APM 1, APM1, GBP28, Gelatin binding protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected in3T3-L1 cells
Planning an IHC experiment? We recommend our IHCeasy Adiponectin Ready-To-Use IHC Kit. Adiponectin primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66239-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66239 targets Adiponectin in IF/ICC applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse
免疫原 Adiponectin fusion protein Ag17383 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 adiponectin, C1Q and collagen domain containing
别名 ACDC, ACRP30, adiponectin, ADIPOQ, ADIPQTL1, ADPN, APM 1, APM1, GBP28, Gelatin binding protein
计算分子量 244 aa, 26 kDa
观测分子量 29 kDa
GenBank蛋白编号BC096308
基因名称 Adiponectin
Gene ID (NCBI) 9370
RRIDAB_2923753
偶联类型 CoraLite®488 Fluorescent Dye
最大激发/发射波长491 nm / 516 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ15848
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Adiponectin (AdipoQ), an adipocyte-derived hormone, is one of the most abundant adipokines in the blood circulation. Adiponectin modulates a number of metabolic processes, including improving insulin sensitivity and anti-inflammatory activity. The role of AdipoQ in reproduction is not yet fully understood, but the expression of AdipoQ in reproductive tissues has been observed in various animals and humans, including chicken testis, bovine ovary, and human placenta. Adiponectin exerts its effects by activating a range of different signaling molecules via binding to two transmembrane AdipoQ receptors, AdipoR1 and AdipoR2. AdipoR1 is expressed primarily in the skeletal muscle, whereas AdipoR2 is predominantly expressed in the liver. AdipoQ May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors.

实验方案

Product Specific Protocols
IF protocol for CL488 Adiponectin antibody CL488-66239Download protocol
Standard Protocols
Click here to view our Standard Protocols