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验证数据展示

  • WB analysis using 67715-1-Ig (same clone as 67715-1-PBS)

    WB analysis using 67715-1-Ig (same clone as 67715-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67715-1-Ig (Alix antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67715-1-PBS in a different storage buffer formulation.

  • WB analysis using 67715-1-Ig (same clone as 67715-1-PBS)

    WB analysis using 67715-1-Ig (same clone as 67715-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67715-1-Ig (Alix antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67715-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 67715-1-Ig (same clone as 67715-1-PBS)

    WB analysis of HEK-293 using 67715-1-Ig (same clone as 67715-1-PBS)

    WB result of Alix antibody (67715-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Alix transfected HEK-293 cells. This data was developed using the same antibody clone with 67715-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 67715-1-Ig (same clone as 67715-1-PBS)

    IHC staining of human stomach cancer using 67715-1-Ig (same clone as 67715-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67715-1-Ig (Alix antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67715-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ALIX antibody included
  • IF Staining of HeLa using 67715-1-Ig (same clone as 67715-1-PBS)

    IF Staining of HeLa using 67715-1-Ig (same clone as 67715-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Alix antibody (67715-1-Ig, Clone: 1H9D9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67715-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

Alix Monoclonal antibody, PBS Only

Alix Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67715-1-PBS

产品说明书

CloneNo. 1H9D9

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

PDCD6IP, Hp95, DRIP4, ALG-2-interacting protein X, ALG-2-interacting protein 1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy ALIX Ready-To-Use IHC Kit. ALIX primary antibody included.

产品信息

67715-1-PBS targets Alix in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Alix fusion protein Ag30437 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 programmed cell death 6 interacting protein
别名 PDCD6IP, Hp95, DRIP4, ALG-2-interacting protein X, ALG-2-interacting protein 1
计算分子量 868 aa, 96 kDa
观测分子量100 kDa
GenBank蛋白编号BC020066
基因名称 Alix
Gene ID (NCBI) 10015
RRIDAB_2882905
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8WUM4
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ALG-2-interacting protein 1 (ALIX), also known as AIP1 or Hp95, is encoded by PDCD6IP gene and is involved in cell death through mechanisms involving its binding partner ALG-2 (apoptosis-linked gene-2). ALG-2 is a 22-kDa protein containing five serially repetitive EF-hand structures and is defined as a regulator of calcium-induced apoptosis following endoplasmic reticulum (ER) stress. ALIX interacts with ALG-2 through its C-terminal proline-rich region and participates in formation of multivesicular bodies. Recent finding suggest that ALIX is a critical component of caspase 9 activation and apoptosis triggered by calcium.