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IHCeasy ALIX Ready-To-Use IHC Kit

ALIX Ready-to-use reagent kit for IHC.

Reactivity

Human, Mouse, Rat

Sample Type

FFPE tissue

Cat No : KHC0873

Synonyms

AIP1, ALG 2 interacting protein 1, Alix, DRIP4, HP95, KIAA1375, PDCD6 interacting protein, PDCD6IP

Validation Data Gallery

  • Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using KHC0873 (ALIX IHC Kit).

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using KHC0873 (ALIX IHC Kit).

  • Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using KHC0873 (ALIX IHC Kit).

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using KHC0873 (ALIX IHC Kit).

  • Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using KHC0873 (ALIX IHC Kit).

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using KHC0873 (ALIX IHC Kit).

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using KHC0873 (ALIX IHC Kit).

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using KHC0873 (ALIX IHC Kit).

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using KHC0873 (ALIX IHC Kit).

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using KHC0873 (ALIX IHC Kit).

  • Components in IHCeasy ready-to-use IHC kit.

Product Information

KHC0873 is a ready-to-use IHC kit for staining of ALIX. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.

Product name IHCeasy ALIX Ready-To-Use IHC Kit
Sample type FFPE tissue
Assay type Immunohistochemistry
Primary antibody type Rabbit Polyclonal
Secondary antibody type Polymer-HRP-Goat anti-Rabbit
Reactivity Human, Mouse, Rat

Kit components

ComponentSizeConcentration
Antigen Retrieval Buffer100 mL50×
Washing Buffer100 mL ×220×
Blocking Buffer5 mLRTU
Primary Antibody5 mLRTU
Secondary Antibody5 mLRTU
Chromogen Component A0.2 mLRTU
Chromogen Component B4 mLRTU
Signal Enhancer5 mLRTU
Counter Staining Reagent5 mLRTU
Mounting Media5 mLRTU
Datasheet1 Copy
Manual1 Copy

Background Information

ALG-2-interacting protein 1 (ALIX), also known as AIP1 or Hp95, is encoded by PDCD6IP gene and is involved in cell death through mechanisms involving its binding partner ALG-2 (apoptosis-linked gene-2). ALG-2 containing five serially repetitive EF-hand structures and is defined as a regulator of calcium-induced apoptosis following endoplasmic reticulum (ER) stress. ALIX interacts with ALG-2 through its C-terminal proline-rich region and participates in formation of multivesicular bodies. Recent finding suggest that ALIX is a critical component of caspase 9 activation and apoptosis triggered by calcium.

Properties

Storage Instructions All the reagents are stored at 2-8°C. The kit is stable for 6 months from the date of receipt.
Synonyms AIP1, ALG 2 interacting protein 1, Alix, DRIP4, HP95, KIAA1375, PDCD6 interacting protein, PDCD6IP