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验证数据展示

  • WB analysis using 66135-1-Ig (same clone as 66135-1-PBS)

    WB analysis using 66135-1-Ig (same clone as 66135-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66135-1-Ig (Alpha 1 Antitrypsin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66135-1-PBS in a different storage buffer formulation.

  • WB analysis using 66135-1-Ig (same clone as 66135-1-PBS)

    WB analysis using 66135-1-Ig (same clone as 66135-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66135-1-Ig (Alpha 1 Antitrypsin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66135-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 66135-1-Ig (same clone as 66135-1-PBS)

    WB analysis of HepG2 using 66135-1-Ig (same clone as 66135-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66135-1-Ig (Alpha 1 Antitrypsin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66135-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 66135-1-Ig (same clone as 66135-1-PBS)

    IHC staining of human liver using 66135-1-Ig (same clone as 66135-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66135-1-Ig (Alpha-1-Antitrypsin Antibody) at dilution of 1:1000 (under 10x lens). This data was developed using the same antibody clone with 66135-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SERPINA1/AAT antibody included
  • IHC staining of human liver using 66135-1-Ig (same clone as 66135-1-PBS)

    IHC staining of human liver using 66135-1-Ig (same clone as 66135-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66135-1-Ig (Alpha-1-Antitrypsin Antibody) at dilution of 1:1000 (under 40x lens). This data was developed using the same antibody clone with 66135-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SERPINA1/AAT antibody included
  • IHC staining of human liver using 66135-1-Ig (same clone as 66135-1-PBS)

    IHC staining of human liver using 66135-1-Ig (same clone as 66135-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66135-1-Ig (Alpha 1 Antitrypsin antibody) at dilution of 1:64000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66135-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SERPINA1/AAT antibody included
  • WB analysis of HepG2 using 66135-1-Ig (same clone as 66135-1-PBS)

    WB analysis of HepG2 using 66135-1-Ig (same clone as 66135-1-PBS)

    WB result of Alpha 1 Antitrypsin antibody (66135-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Alpha 1 Antitrypsin transfected HepG2 cells. This data was developed using the same antibody clone with 66135-1-PBS in a different storage buffer formulation.

  • IP experiment of human plasma using 66135-1-Ig (same clone as 66135-1-PBS)

    IP experiment of human plasma using 66135-1-Ig (same clone as 66135-1-PBS)

    IP result of anti-Alpha 1 Antitrypsin (IP:66135-1-Ig, 5ug; Detection:66135-1-Ig 1:1000) with human plasma lysate 4000ug. This data was developed using the same antibody clone with 66135-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66135-1-Ig (same clone as 66135-1-PBS)

    IF Staining of HepG2 using 66135-1-Ig (same clone as 66135-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Alpha 1 Antitrypsin antibody (66135-1-Ig, Clone: 1A9G6 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 66135-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 66135-1-Ig (same clone as 66135-1-PBS)

    FC experiment of HepG2 using 66135-1-Ig (same clone as 66135-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human Alpha 1 Antitrypsin (66135-1-Ig, Clone:1A9G6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (black). Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100. This data was developed using the same antibody clone with 66135-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

Alpha-1-Antitrypsin Monoclonal antibody, PBS Only

Alpha-1-Antitrypsin Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 66135-1-PBS

产品说明书

CloneNo. 1A9G6

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

别名

a1-antitrypsin, Serpin A1, SERPINA1, AAT, A1AT

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy SERPINA1/AAT Ready-To-Use IHC Kit. SERPINA1/AAT primary antibody included.

产品信息

66135-1-PBS targets Alpha-1-Antitrypsin in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 Alpha-1-Antitrypsin fusion protein Ag9516 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1
别名 a1-antitrypsin, Serpin A1, SERPINA1, AAT, A1AT
计算分子量 418 aa, 47 kDa
观测分子量 51 kDa
GenBank蛋白编号BC015642
基因名称 Alpha 1-Antitrypsin
Gene ID (NCBI) 5265
RRIDAB_2881534
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP01009
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SERPINA1 is the gene for a protein called alpha-1-antitrypsin (AAT), which is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. AAT is a glycoprotein synthesized primarily by hepatocytes, with smaller amountssynthesized by intestinal epithelial cells, neutrophils, pulmonary alveolar cells and macrophages. AAT is the most abundant, endogenous serine protease inhibitor in blood circulation and it has been implicated in regulating vital fluid phase biological events such as blood coagulation, fibrinolysis, complement activation, apoptosis, reproduction, tumor progression and inflammatory response. The primary function of AAT is thought to be the inactivation of neutrophil elastase and other endogenous serine proteases. Defects in SERPINA1 can cause emphysema or liver disease.