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验证数据展示

  • IF Staining of HepG2 using CL488-66135

    IF Staining of HepG2 using CL488-66135

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488-conjugated Alpha 1 Antitrypsin antibody (CL488-66135, Clone: 1A9G6 ) at dilution of 1:400.

CoraLite® Plus 488-conjugated Alpha-1-Antitrypsin Monoclonal antibody

Alpha-1-Antitrypsin Monoclonal Antibody for IF/ICC
Cat No. CL488-66135
CloneNo. 1A9G6

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC

Synonyms

a1-antitrypsin, Serpin A1, SERPINA1, AAT, A1AT

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy SERPINA1/AAT Ready-To-Use IHC Kit. SERPINA1/AAT primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66135-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66135 targets Alpha-1-Antitrypsin in IF/ICC applications and shows reactivity with human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human
Immunogen Alpha-1-Antitrypsin fusion protein Ag9516 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1
Synonyms a1-antitrypsin, Serpin A1, SERPINA1, AAT, A1AT
Calculated Molecular Weight 418 aa, 47 kDa
Observed Molecular Weight 51 kDa
GenBank Accession NumberBC015642
Gene Symbol Alpha 1-Antitrypsin
Gene ID (NCBI) 5265
RRIDAB_2883270
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP01009
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SERPINA1 is the gene for a protein called alpha-1-antitrypsin (AAT), which is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. AAT is a glycoprotein synthesized primarily by hepatocytes, with smaller amountssynthesized by intestinal epithelial cells, neutrophils, pulmonary alveolar cells and macrophages. AAT is the most abundant, endogenous serine protease inhibitor in blood circulation and it has been implicated in regulating vital fluid phase biological events such as blood coagulation, fibrinolysis, complement activation, apoptosis, reproduction, tumor progression and inflammatory response. The primary function of AAT is thought to be the inactivation of neutrophil elastase and other endogenous serine proteases. Defects in SERPINA1 can cause emphysema or liver disease.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Alpha-1-Antitrypsin antibody CL488-66135Download protocol
Standard Protocols
Click here to view our Standard Protocols