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验证数据展示

  • IF Staining of rat heart using CL488-11313

    IF Staining of rat heart using CL488-11313

    Immunofluorescent analysis of (4% PFA) fixed rat heart tissue using CoraLite® Plus 488 Alpha Actinin antibody (CL488-11313) at dilution of 1:200.

  • IF Staining of rat heart using CL488-11313

    IF Staining of rat heart using CL488-11313

    Immunofluorescent analysis of (4% PFA) fixed rat heart tissue using CoraLite® Plus 488 Alpha Actinin antibody (CL488-11313) at dilution of 1:200.

  • FC experiment of A431 using CL488-11313

    FC experiment of A431 using CL488-11313

    1X10^6 A431 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human Alpha Actinin (CL488-11313) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated Alpha Actinin Polyclonal antibody

Alpha Actinin Polyclonal Antibody for IF-P, FC (Intra)
Cat No. CL488-11313

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF-P, FC (Intra)

别名

Alpha actinin 1, ACTN1, Alpha-actinin cytoskeletal isoform, ACTN 1, actinin, alpha 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inrat heart tissue
Positive FC (Intra) detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy Alpha Actinin Ready-To-Use IHC Kit. Alpha Actinin primary antibody included.

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-11313 targets Alpha Actinin in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF-P, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 Alpha Actinin fusion protein Ag1859 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 actinin, alpha 1
别名 Alpha actinin 1, ACTN1, Alpha-actinin cytoskeletal isoform, ACTN 1, actinin, alpha 1
计算分子量 103 kDa
观测分子量 100-105 kDa
GenBank蛋白编号BC015766
基因名称 Alpha Actinin
Gene ID (NCBI) 87
RRIDAB_3083899
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP12814
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Alpha Actinins are highly conserved actin-binding proteins that form antiparallel homodimers with the actin-binding domains on each end of the molecule, allowing crosslinking of actin molecules. There are four alpha actinin isoforms identified: two ubiquitously expressed cytoskeletal isoforms (ACTN1 and ACTN4) and two striated muscle-specific sarcomeric isoforms (ACTN2 and ACTN3). ACTN1 is also alternatively spliced to form smooth muscle or brain specific isoforms. This antibody recognize endogenous ACTN1, while it may cross-react with ACTN2 and ACTN4.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Alpha Actinin antibody CL488-11313Download protocol
FC protocol for CL Plus 488 Alpha Actinin antibody CL488-11313Download protocol
Standard Protocols
Click here to view our Standard Protocols