验证数据展示
经过测试的应用
| Positive IF/ICC detected in | C2C12 cells |
Planning an IHC experiment? We recommend our IHCeasy Alpha Actinin Ready-To-Use IHC Kit. Alpha Actinin primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66895-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-66895 targets Alpha Actinin in IF/ICC applications and shows reactivity with Human, rat, mouse samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | Human, rat, mouse |
| 免疫原 | Alpha Actinin fusion protein Ag28383 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | actinin, alpha 1 |
| 别名 | actinin, alpha 1, ACTN1, Alpha Actinin, Alpha actinin 1, F actin cross linking protein, FLJ40884, FLJ54432, Non muscle alpha actinin 1 |
| 计算分子量 | 103 kDa |
| 观测分子量 | 100-105 kDa |
| GenBank蛋白编号 | BC015766 |
| 基因名称 | Alpha Actinin |
| Gene ID (NCBI) | 87 |
| RRID | AB_3084734 |
| 偶联类型 | CoraLite®594 Fluorescent Dye |
| 最大激发/发射波长 | 588 nm / 604 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P12814 |
| 储存缓冲液 | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Alpha Actinins are highly conserved actin-binding proteins that form antiparallel homodimers with the actin-binding domains on each end of the molecule, allowing crosslinking of actin molecules. There are four alpha actinin isoforms identified: two ubiquitously expressed cytoskeletal isoforms (ACTN1 and ACTN4) and two striated muscle-specific sarcomeric isoforms (ACTN2 and ACTN3). ACTN1 is also alternatively spliced to form smooth muscle or brain specific isoforms. This antibody recognize endogenous ACTN1, while it may cross-react with ACTN2 and ACTN4.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 Alpha Actinin antibody CL594-66895 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |