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Alpha Antichymotrypsin Monoclonal antibody, PBS Only

Alpha Antichymotrypsin Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3F2F10

Cat No : 66078-1-PBS

Print datasheet

Synonyms

A1 Antichymotrypsin, A-1-Antichymotrypsin, AACT, ACT, Alpha 1 antichymotrypsin, Alpha Antichymotrypsin, GIG24, GIG25, Serpin A3, SERPINA3

验证数据展示

  • WB analysis of human plasma using 66078-1-Ig (same clone as 66078-1-PBS)

    WB analysis of human plasma using 66078-1-Ig (same clone as 66078-1-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 66078-1-Ig (Alpha Antichymotrypsin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66078-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 66078-1-Ig (same clone as 66078-1-PBS)

    IHC staining of human tonsillitis using 66078-1-Ig (same clone as 66078-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66078-1-Ig (alpha 1 Antichymotrypsin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66078-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SERPINA3/AACT antibody included
  • IHC staining of human tonsillitis using 66078-1-Ig (same clone as 66078-1-PBS)

    IHC staining of human tonsillitis using 66078-1-Ig (same clone as 66078-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66078-1-Ig (alpha 1 Antichymotrypsin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66078-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SERPINA3/AACT antibody included
  • IF Staining of human tonsillitis using 66078-1-Ig (same clone as 66078-1-PBS)

    IF Staining of human tonsillitis using 66078-1-Ig (same clone as 66078-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 66078-1-Ig (alpha 1 Antichymotrypsin antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66078-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy SERPINA3/AACT Ready-To-Use IHC Kit. SERPINA3/AACT primary antibody included.

产品信息

66078-1-PBS targets Alpha Antichymotrypsin in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, pig samples.

Tested Applications WB, IHC, IF-P, Indirect ELISA Application Description
Tested Reactivity human, pig
Immunogen Alpha Antichymotrypsin fusion protein Ag2830 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3
Synonyms A1 Antichymotrypsin, A-1-Antichymotrypsin, AACT, ACT, Alpha 1 antichymotrypsin, Alpha Antichymotrypsin, GIG24, GIG25, Serpin A3, SERPINA3
Calculated Molecular Weight 448 aa, 48 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC010530
Gene Symbol Alpha 1 Antichymotrypsin
Gene ID (NCBI) 12
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP01011
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Human SerpinA3, also known as Alpha 1-antichymotrypsin (AACT), is a member of the serine protease inhibitor class, and is a plasma alpha globulin glycoprotein which increases in the blood during the inflammatory process. SerpinA3, is also an inhibitor of neutrophil cathepsin G, mast cell chymases and pancreatic chymotrypsin. SerpinA3 is produced primarily in the liver, and is identified as an acute-phase inflammatory protein. SerpinA3 deficiency has been associated with liver disease. SerpinA3 has also been implicated in the pathology of a number of devastating human diseases including chronic obstructive pulmonary disease (COPD), Parkinson's disease (PD), Alzheimer's disease (AD), Stroke, Cystic Fibrosis, Cerebral Haemorrhage and Multiple System Atrophy.