验证数据展示
经过测试的应用
| Positive WB detected in | human plasma, human colon tissue |
| Positive IHC detected in | human tonsillitis tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human tonsillitis tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IHC | See 2 publications below |
产品信息
66078-1-Ig targets Alpha 1 antichymotrypsin in WB, IHC, IF-P, ELISA applications and shows reactivity with human, pig samples.
| 经测试应用 | WB, IHC, IF-P, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, pig |
| 文献引用反应性 | human |
| 免疫原 | Alpha 1 antichymotrypsin fusion protein Ag2830 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3 |
| 别名 | Alpha Antichymotrypsin, SERPINA3, ACT, AACT, A-1-Antichymotrypsin |
| 计算分子量 | 448 aa, 48 kDa |
| 观测分子量 | 60 kDa |
| GenBank蛋白编号 | BC010530 |
| 基因名称 | Alpha 1 Antichymotrypsin |
| Gene ID (NCBI) | 12 |
| RRID | AB_11182502 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P01011 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Human SerpinA3, also known as Alpha 1-antichymotrypsin (AACT), is a member of the serine protease inhibitor class, and is a plasma alpha globulin glycoprotein which increases in the blood during the inflammatory process. SerpinA3, is also an inhibitor of neutrophil cathepsin G, mast cell chymases and pancreatic chymotrypsin. SerpinA3 is produced primarily in the liver, and is identified as an acute-phase inflammatory protein. SerpinA3 deficiency has been associated with liver disease. SerpinA3 has also been implicated in the pathology of a number of devastating human diseases including chronic obstructive pulmonary disease (COPD), Parkinson's disease (PD), Alzheimer's disease (AD), Stroke, Cystic Fibrosis, Cerebral Haemorrhage and Multiple System Atrophy.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Alpha 1 antichymotrypsin antibody 66078-1-Ig | Download protocol |
| IHC protocol for Alpha 1 antichymotrypsin antibody 66078-1-Ig | Download protocol |
| IF protocol for Alpha 1 antichymotrypsin antibody 66078-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Br J Cancer Identification of GlcNAcylated alpha-1-antichymotrypsin as an early biomarker in human non-small-cell lung cancer by quantitative proteomic analysis with two lectins. | ||
Breast Cancer Overexpression of SERPINA3 promotes tumor invasion and migration, epithelial-mesenchymal-transition in triple-negative breast cancer cells. | ||
Pharmaceutics Distribution of Monocarboxylate Transporters in Brain and Choroid Plexus Epithelium |
