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验证数据展示

  • FC experiment of MCF-7 using CL594-66221

    FC experiment of MCF-7 using CL594-66221

    1X10^6 MCF-7 cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human Alpha E-Catenin (CL594-66221, Clone:2B7B8) (red), or 0.8 ug CoraLite®594 Mouse IgG1 Isotype Control (MOPC-21) (CL594-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite®594-conjugated Alpha E-Catenin Monoclonal antibody

Alpha E-Catenin Monoclonal Antibody for FC (Intra)
Cat No. CL594-66221

Print datasheet

CloneNo. 2B7B8

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

FC (Intra)

Synonyms

Alpha E catenin, Alpha E-Catenin, Cadherin associated protein, CAP102, Catenin alpha 1, CTNNA1, FLJ36832, FLJ52416

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inMCF-7 cells
Positive FC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy CTNNA1 Ready-To-Use IHC Kit. CTNNA1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66221-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66221 targets Alpha E-Catenin in FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human, mouse
Immunogen Alpha E-Catenin fusion protein Ag23603 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name catenin (cadherin-associated protein), alpha 1, 102kDa
Synonyms Alpha E catenin, Alpha E-Catenin, Cadherin associated protein, CAP102, Catenin alpha 1, CTNNA1, FLJ36832, FLJ52416
Calculated Molecular Weight 906 aa, 100 kDa
Observed Molecular Weight 95-100 kDa
GenBank Accession NumberBC031262
Gene Symbol Alpha E-Catenin
Gene ID (NCBI) 1495
RRIDAB_3084704
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP35221
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Alpha catenin is an essential component of adherens junctions that connects E-cadherin-β-catenin complexes with the actin cytoskeleton. It also recruits a range of other important proteins to developing intercellular junctions. Three alpha catenins exist in human: alpha-E-catenin, alpha-N-catenin, and alpha-T-catenin, which share substantial amino-acid sequence similarity but have distinct tissue distribution. alpha-E-catenin is ubiquitously expressed, alpha-N-catenin is restricted to neuronal tissue, and alpha-T-catenin is primarily expressed in heart tissue. Reduced levels of alpha-E-catenin protein seem to be characteristic of many different human cancers, including malignant tumours of the breast, colon, stomach, oesophagus, bladder and liver. In addition, the loss of alpha-E-catenin often correlates with the degree of tumour differentiation and metastasis.

实验方案

Product Specific Protocols
FC protocol for CL594 Alpha E-Catenin antibody CL594-66221Download protocol
Standard Protocols
Click here to view our Standard Protocols