Annexin A11 Monoclonal antibody

Annexin A11 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1A3C4

Cat no : 68089-1-Ig

Print datasheet

Synonyms

ANXA11, ANX11, Annexin-11, Annexin XI, Annexin 11



经过测试的应用

Positive WB detected inA431 cells, A549 cells, HeLa cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, pig heart tissue, rabbit heart
Positive IHC detected inhuman lung cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHEK-293T cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy ANXA11 Ready-To-Use IHC Kit. ANXA11 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68089-1-Ig targets Annexin A11 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat, pig, rabbit
Cited Reactivityhuman, mouse
Immunogen Annexin A11 fusion protein Ag31839 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name annexin A11
Synonyms ANXA11, ANX11, Annexin-11, Annexin XI, Annexin 11
Calculated Molecular Weight 56 kDa
Observed Molecular Weight 56 kDa
GenBank Accession NumberBC007564
Gene Symbol Annexin A11
Gene ID (NCBI) 311
RRIDAB_2918826
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP50995
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Annexin A11 (Anxa11) is one member of annexins that are Ca2+-regulated phospholipid-binding proteins. Annexin A11 plays an important role in cell division, differentiation, apoptosis, vesicle trafficking and Ca2+ signaling. Annexin A11 dysregulation is involved in the progression, drug-resistance, recurrence of systemic autoimmune disease and cancer.

实验方案

Product Specific Protocols
WB protocol for Annexin A11 antibody 68089-1-IgDownload protocol
IHC protocol for Annexin A11 antibody 68089-1-IgDownload protocol
IF protocol for Annexin A11 antibody 68089-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Mol Cell Proteomics

FMRP long-range transport and degradation are mediated by Dynlrb1 in sensory neurons

Authors - Sara Emad El-Agamy
humanWB,IHC

Acta Neuropathol

Annexin A11 aggregation in FTLD-TDP type C and related neurodegenerative disease proteinopathies

Authors - John L Robinson