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Annexin A11 Monoclonal antibody, PBS Only

Annexin A11 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

rabbit, pig, rat, mouse, human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1A3C4

Cat no : 68089-1-PBS

Print datasheet

Synonyms

ANXA11, ANX11, Annexin-11, Annexin XI, Annexin 11

验证数据展示

  • WB analysis using 68089-1-Ig (same clone as 68089-1-PBS)

    WB analysis using 68089-1-Ig (same clone as 68089-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68089-1-Ig (Annexin A11 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68089-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 68089-1-Ig (same clone as 68089-1-PBS)

    IHC staining of human lung cancer using 68089-1-Ig (same clone as 68089-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68089-1-Ig (Annexin A11 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68089-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ANXA11 antibody included
  • IHC staining of human lung cancer using 68089-1-Ig (same clone as 68089-1-PBS)

    IHC staining of human lung cancer using 68089-1-Ig (same clone as 68089-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68089-1-Ig (Annexin A11 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68089-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ANXA11 antibody included
  • FC experiment of HEK-293T using 68089-1-Ig (same clone as 68089-1-PBS)

    FC experiment of HEK-293T using 68089-1-Ig (same clone as 68089-1-PBS)

    1X10^6 HEK-293T cells were intracellularly stained with 0.4 ug Anti-Human Annexin A11 (68089-1-Ig, Clone:1A3C4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68089-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 68089-1-Ig (same clone as 68089-1-PBS)

    FC experiment of HeLa using 68089-1-Ig (same clone as 68089-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Annexin A11 (68089-1-Ig, Clone:1A3C4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68089-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 68089-1-Ig (same clone as 68089-1-PBS)

    IF Staining of HeLa using 68089-1-Ig (same clone as 68089-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Annexin A11 antibody (68089-1-Ig, Clone: 1A3C4 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68089-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy ANXA11 Ready-To-Use IHC Kit. ANXA11 primary antibody included.

产品信息

68089-1-PBS targets Annexin A11 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with rabbit, pig, rat, mouse, human samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity rabbit, pig, rat, mouse, human
Immunogen Annexin A11 fusion protein Ag31839 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name annexin A11
Synonyms ANXA11, ANX11, Annexin-11, Annexin XI, Annexin 11
Calculated Molecular Weight 56 kDa
GenBank Accession NumberBC007564
Gene Symbol Annexin A11
Gene ID (NCBI) 311
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP50995
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Annexin A11 (Anxa11) is one member of annexins that are Ca2+-regulated phospholipid-binding proteins. Annexin A11 plays an important role in cell division, differentiation, apoptosis, vesicle trafficking and Ca2+ signaling. Annexin A11 dysregulation is involved in the progression, drug-resistance, recurrence of systemic autoimmune disease and cancer.