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Annexin A4 Monoclonal antibody, PBS Only

Annexin A4 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2c

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A10B5

Cat no : 60288-2-PBS

Print datasheet

Synonyms

35-beta calcimedin, Annexin A4, Annexin IV, Annexin-4, ANXA4, Chromobindin-4, Endonexin I, Lipocortin IV

验证数据展示

  • WB analysis of COLO 320 using 60288-2-Ig (same clone as 60288-2-PBS)

    WB analysis of COLO 320 using 60288-2-Ig (same clone as 60288-2-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 60288-2-Ig (Annexin IV antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60288-2-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 60288-2-Ig (same clone as 60288-2-PBS)

    IHC staining of human placenta using 60288-2-Ig (same clone as 60288-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60288-2-Ig (Annexin IV antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60288-2-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 60288-2-Ig (same clone as 60288-2-PBS)

    IHC staining of human placenta using 60288-2-Ig (same clone as 60288-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60288-2-Ig (Annexin IV antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60288-2-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 60288-2-Ig (same clone as 60288-2-PBS)

    IF Staining of HeLa using 60288-2-Ig (same clone as 60288-2-PBS)

    Immunofluorescent analysis of (10% Formaldehyde ) fixed HeLa cells using 60288-2-Ig(Annexin IV antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60288-2-PBS in a different storage buffer formulation.

产品信息

60288-2-PBS targets Annexin A4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen Annexin A4 fusion protein Ag0130 种属同源性预测
Host / Isotype Mouse / IgG2c
Class Monoclonal
Type Antibody
Full Name annexin A4
Synonyms 35-beta calcimedin, Annexin A4, Annexin IV, Annexin-4, ANXA4, Chromobindin-4, Endonexin I, Lipocortin IV
Calculated Molecular Weight 36 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC000182
Gene Symbol Annexin A4
Gene ID (NCBI) 307
RRIDAB_2881405
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP09525
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Annexin A4 (Anxa4) is one of the Ca2 +-regulated and phospholipid-binding annexin superfamily proteins, and is located at 2p13. Anxa4 has a potential role in diagnosis, prognosis, and treatment of certain cancers. Annexin A4 shares 45 to 59% identity with other members and possibly interacts with ATP. Annexin A4 has in vitro anticoagulant activity and also inhibits phospholipase A2 activity. The Annexin A4 protein is expressed in a wide range of tissues, associated with polarized epithelial cells and is proposed to be involved in the regulation of chloride ions across the epithelial membrane.