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Annexin A4 Monoclonal antibody, PBS Only

Annexin A4 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2c

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A10B5

Cat no : 60288-2-PBS

Print datasheet

Synonyms

35-beta calcimedin, Annexin A4, Annexin IV, Annexin-4, ANXA4, Chromobindin-4, Endonexin I, Lipocortin IV

验证数据展示

  • WB analysis of COLO 320 using 60288-2-Ig (same clone as 60288-2-PBS)

    WB analysis of COLO 320 using 60288-2-Ig (same clone as 60288-2-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 60288-2-Ig (Annexin IV antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60288-2-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 60288-2-Ig (same clone as 60288-2-PBS)

    IHC staining of human placenta using 60288-2-Ig (same clone as 60288-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60288-2-Ig (Annexin IV antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60288-2-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 60288-2-Ig (same clone as 60288-2-PBS)

    IHC staining of human placenta using 60288-2-Ig (same clone as 60288-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60288-2-Ig (Annexin IV antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60288-2-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 60288-2-Ig (same clone as 60288-2-PBS)

    IF Staining of HeLa using 60288-2-Ig (same clone as 60288-2-PBS)

    Immunofluorescent analysis of (10% Formaldehyde ) fixed HeLa cells using 60288-2-Ig(Annexin IV antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60288-2-PBS in a different storage buffer formulation.

产品信息

60288-2-PBS targets Annexin A4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen Annexin A4 fusion protein Ag0130 种属同源性预测
Host / Isotype Mouse / IgG2c
Class Monoclonal
Type Antibody
Full Name annexin A4
Synonyms 35-beta calcimedin, Annexin A4, Annexin IV, Annexin-4, ANXA4, Chromobindin-4, Endonexin I, Lipocortin IV
Calculated Molecular Weight 36 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC000182
Gene Symbol Annexin A4
Gene ID (NCBI) 307
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP09525
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Annexin A4 (Anxa4) is one of the Ca2 +-regulated and phospholipid-binding annexin superfamily proteins, and is located at 2p13. Anxa4 has a potential role in diagnosis, prognosis, and treatment of certain cancers. Annexin A4 shares 45 to 59% identity with other members and possibly interacts with ATP. Annexin A4 has in vitro anticoagulant activity and also inhibits phospholipase A2 activity. The Annexin A4 protein is expressed in a wide range of tissues, associated with polarized epithelial cells and is proposed to be involved in the regulation of chloride ions across the epithelial membrane.