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Annexin A4 Monoclonal antibody

Annexin A4 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2c

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1A10B5

Cat no : 60288-2-Ig

Print datasheet

Synonyms

35-beta calcimedin, Annexin A4, Annexin IV, Annexin-4, ANXA4, Chromobindin-4, Endonexin I, Lipocortin IV

验证数据展示

  • WB analysis of COLO 320 using 60288-2-Ig

    WB analysis of COLO 320 using 60288-2-Ig

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 60288-2-Ig (Annexin IV antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 60288-2-Ig

    IHC staining of human placenta using 60288-2-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60288-2-Ig (Annexin IV antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 60288-2-Ig

    IHC staining of human placenta using 60288-2-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60288-2-Ig (Annexin IV antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 60288-2-Ig

    IF Staining of HeLa using 60288-2-Ig

    Immunofluorescent analysis of (10% Formaldehyde ) fixed HeLa cells using 60288-2-Ig(Annexin IV antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inCOLO 320 cells, human placenta tissue, A549 cells, human heart tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60288-2-Ig targets Annexin A4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen Annexin A4 fusion protein Ag0130 种属同源性预测
Host / Isotype Mouse / IgG2c
Class Monoclonal
Type Antibody
Full Name annexin A4
Synonyms 35-beta calcimedin, Annexin A4, Annexin IV, Annexin-4, ANXA4, Chromobindin-4, Endonexin I, Lipocortin IV
Calculated Molecular Weight 36 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC000182
Gene Symbol Annexin A4
Gene ID (NCBI) 307
RRIDAB_2881405
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP09525
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Annexin A4 (Anxa4) is one of the Ca2 +-regulated and phospholipid-binding annexin superfamily proteins, and is located at 2p13. Anxa4 has a potential role in diagnosis, prognosis, and treatment of certain cancers. Annexin A4 shares 45 to 59% identity with other members and possibly interacts with ATP. Annexin A4 has in vitro anticoagulant activity and also inhibits phospholipase A2 activity. The Annexin A4 protein is expressed in a wide range of tissues, associated with polarized epithelial cells and is proposed to be involved in the regulation of chloride ions across the epithelial membrane.

实验方案

Product Specific Protocols
WB protocol for Annexin A4 antibody 60288-2-IgDownload protocol
IHC protocol for Annexin A4 antibody 60288-2-IgDownload protocol
IF protocol for Annexin A4 antibody 60288-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols