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Annexin VI Monoclonal antibody

Annexin VI Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

2H7G7

Cat no : 68086-1-Ig

Print datasheet

Synonyms

Annexin A6, ANXA6, ANX6, Annexin-6, Annexin 6

验证数据展示

  • WB analysis using 68086-1-Ig

    WB analysis using 68086-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68086-1-Ig (Annexin VI antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IF Staining of HepG2 using 68086-1-Ig

    IF Staining of HepG2 using 68086-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Annexin VI antibody (68086-1-Ig, Clone: 2H7G7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • FC experiment of K-562 using 68086-1-Ig

    FC experiment of K-562 using 68086-1-Ig

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human Annexin VI (68086-1-Ig, Clone:2H7G7) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inLNCaP cells, HeLa cells, human placenta tissue, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inK-562 cells
Planning an IHC experiment? We recommend our IHCeasy ANXA6 Ready-To-Use IHC Kit. ANXA6 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68086-1-Ig targets Annexin VI in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Applications WB, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat, pig, rabbit
Immunogen Annexin VI fusion protein Ag3263 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name annexin A6
Synonyms Annexin A6, ANXA6, ANX6, Annexin-6, Annexin 6
Calculated Molecular Weight 673 aa, 76 kDa
Observed Molecular Weight67 kDa
GenBank Accession NumberBC017046
Gene Symbol Annexin VI
Gene ID (NCBI) 309
RRIDAB_2918823
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP08133
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ANXA6, also called CBP68, or 67 kDa calelectrin, belongs to a family of calcium-dependent membrane and phospholipid binding proteins. Several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. The annexin VI gene is approximately 60 kbp long and contains 26 exons. It encodes a protein of about 68 kDa that consists of eight 68-amino acid repeats separated by linking sequences of variable lengths. It is highly similar to human annexins I and II sequences, each of which contain four such repeats. Annexin VI has been implicated in mediating the endosome aggregation and vesicle fusion in secreting epithelia during exocytosis.

实验方案

Product Specific Protocols
WB protocol for Annexin VI antibody 68086-1-IgDownload protocol
IF protocol for Annexin VI antibody 68086-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols