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验证数据展示

  • WB analysis using 68086-1-Ig

    WB analysis using 68086-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68086-1-Ig (Annexin VI antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IF Staining of HepG2 using 68086-1-Ig

    IF Staining of HepG2 using 68086-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Annexin VI antibody (68086-1-Ig, Clone: 2H7G7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • FC experiment of K-562 using 68086-1-Ig

    FC experiment of K-562 using 68086-1-Ig

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human Annexin VI (68086-1-Ig, Clone:2H7G7) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Annexin VI Monoclonal antibody

Annexin VI Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 68086-1-Ig

产品说明书

CloneNo. 2H7G7

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, IF/ICC, FC (Intra), ELISA

别名

Annexin A6, ANXA6, ANX6, Annexin-6, Annexin 6

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, HeLa cells, human placenta tissue, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inK-562 cells
Planning an IHC experiment? We recommend our IHCeasy ANXA6 Ready-To-Use IHC Kit. ANXA6 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68086-1-Ig targets Annexin VI in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原 Annexin VI fusion protein Ag3263 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 annexin A6
别名 Annexin A6, ANXA6, ANX6, Annexin-6, Annexin 6
计算分子量 673 aa, 76 kDa
观测分子量67 kDa
GenBank蛋白编号BC017046
基因名称 Annexin VI
Gene ID (NCBI) 309
RRIDAB_2918823
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP08133
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ANXA6, also called CBP68, or 67 kDa calelectrin, belongs to a family of calcium-dependent membrane and phospholipid binding proteins. Several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. The annexin VI gene is approximately 60 kbp long and contains 26 exons. It encodes a protein of about 68 kDa that consists of eight 68-amino acid repeats separated by linking sequences of variable lengths. It is highly similar to human annexins I and II sequences, each of which contain four such repeats. Annexin VI has been implicated in mediating the endosome aggregation and vesicle fusion in secreting epithelia during exocytosis.

实验方案

Product Specific Protocols
WB protocol for Annexin VI antibody 68086-1-IgDownload protocol
IF protocol for Annexin VI antibody 68086-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols