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Annexin VI Monoclonal antibody, PBS Only

Annexin VI Monoclonal Antibody for WB, IF, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, rabbit, pig

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2H7G7

Cat No : 68086-1-PBS

Print datasheet

Synonyms

67 kDa calelectrin, Annexin 6, annexin A6, Annexin VI, ANX6, ANXA6, Calphobindin II, CBP68, Chromobindin 20, CPB II, Lipocortin VI, p68, p70, Protein III

验证数据展示

  • WB analysis using 68086-1-Ig (same clone as 68086-1-PBS)

    WB analysis using 68086-1-Ig (same clone as 68086-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68086-1-Ig (Annexin VI antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68086-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 68086-1-Ig (same clone as 68086-1-PBS)

    IF Staining of HepG2 using 68086-1-Ig (same clone as 68086-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Annexin VI antibody (68086-1-Ig, Clone: 2H7G7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68086-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy ANXA6 Ready-To-Use IHC Kit. ANXA6 primary antibody included.

产品信息

68086-1-PBS targets Annexin VI in WB, IF, Indirect ELISA applications and shows reactivity with Human, mouse, rat, rabbit, pig samples.

Tested Applications WB, IF, Indirect ELISA Application Description
Tested Reactivity Human, mouse, rat, rabbit, pig
Immunogen Annexin VI fusion protein Ag3263 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name annexin A6
Synonyms 67 kDa calelectrin, Annexin 6, annexin A6, Annexin VI, ANX6, ANXA6, Calphobindin II, CBP68, Chromobindin 20, CPB II, Lipocortin VI, p68, p70, Protein III
Calculated Molecular Weight 673 aa, 76 kDa
Observed Molecular Weight67 kDa
GenBank Accession NumberBC017046
Gene Symbol ANXA6
Gene ID (NCBI) 309
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP08133
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

ANXA6, also called CBP68, or 67 kDa calelectrin, belongs to a family of calcium-dependent membrane and phospholipid binding proteins. Several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. The annexin VI gene is approximately 60 kbp long and contains 26 exons. It encodes a protein of about 68 kDa that consists of eight 68-amino acid repeats separated by linking sequences of variable lengths. It is highly similar to human annexins I and II sequences, each of which contain four such repeats. Annexin VI has been implicated in mediating the endosome aggregation and vesicle fusion in secreting epithelia during exocytosis.