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Apolipoprotein A II Monoclonal antibody, PBS Only

Apolipoprotein A II Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G1A2

Cat no : 66773-1-PBS

Print datasheet

Synonyms

Apo AII, ApoA II, APOA2, apolipoprotein A II, Apolipoprotein A2

验证数据展示

  • WB analysis of human plasma using 66773-1-Ig (same clone as 66773-1-PBS)

    WB analysis of human plasma using 66773-1-Ig (same clone as 66773-1-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 66773-1-Ig (APOA2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66773-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66773-1-Ig (same clone as 66773-1-PBS)

    IHC staining of human liver cancer using 66773-1-Ig (same clone as 66773-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66773-1-Ig (Apolipoprotein A II antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66773-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with APOA2 antibody included
  • IHC staining of human liver cancer using 66773-1-Ig (same clone as 66773-1-PBS)

    IHC staining of human liver cancer using 66773-1-Ig (same clone as 66773-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66773-1-Ig (Apolipoprotein A II antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66773-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with APOA2 antibody included
Planning an IHC experiment? We recommend our IHCeasy APOA2 Ready-To-Use IHC Kit. APOA2 primary antibody included.

产品信息

66773-1-PBS targets Apolipoprotein A II in WB, IHC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity Human
Immunogen Apolipoprotein A II fusion protein Ag9863 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name apolipoprotein A-II
Synonyms Apo AII, ApoA II, APOA2, apolipoprotein A II, Apolipoprotein A2
Calculated Molecular Weight 100 aa, 11 kDa
Observed Molecular Weight 8-11 kDa
GenBank Accession NumberBC005282
Gene Symbol APOA2
Gene ID (NCBI) 336
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP02652
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Apolipoprotein A-II (APOA2) is a major component of high density lipoprotein (HDL) and it plays an important role in directing the fate of lipid metabolism among HDL. It is primarily synthesized by liver. The predicted MW of ApoA2 is 11 kDa, while the mature form is smaller (7-10 kDa) since the signal peptide was cleaved. In humans, most of circulating apoA2 exist as dimer. Five types of APOA2 dimer exist: homodimer apoA2-ATQ/ATQ, heterodimer apoA2-ATQ/AT, homodimer apoA2-AT/AT, apoA2-AT/A, and apoA2-A/A. ApoA2 isoforms are considered to be some of the most promising serum/plasma biomarkers for aiding the early detection of pancreatic cancer. (PMID: 29081441, 29481802)