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Apolipoprotein AI Monoclonal antibody, PBS Only

Apolipoprotein AI Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C9G5

Cat no : 66206-1-PBS

Print datasheet

Synonyms

APOA1, apolipoprotein A I, Apo-AI, ApoAI, ApoA I

验证数据展示

  • WB analysis of human plasma using 66206-1-Ig (same clone as 66206-1-PBS)

    WB analysis of human plasma using 66206-1-Ig (same clone as 66206-1-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 66206-1-Ig (APOA1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66206-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66206-1-Ig (same clone as 66206-1-PBS)

    IHC staining of human liver cancer using 66206-1-Ig (same clone as 66206-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66206-1-Ig (APOA1 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66206-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with APOA1 antibody included
  • IHC staining of human liver cancer using 66206-1-Ig (same clone as 66206-1-PBS)

    IHC staining of human liver cancer using 66206-1-Ig (same clone as 66206-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66206-1-Ig (APOA1 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66206-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with APOA1 antibody included
  • IF Staining of HepG2 using 66206-1-Ig (same clone as 66206-1-PBS)

    IF Staining of HepG2 using 66206-1-Ig (same clone as 66206-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66206-1-Ig (APOA1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66206-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 66206-1-Ig (same clone as 66206-1-PBS)

    FC experiment of HepG2 using 66206-1-Ig (same clone as 66206-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human Apolipoprotein AI (66206-1-Ig, Clone:1C9G5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66206-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy APOA1 Ready-To-Use IHC Kit. APOA1 primary antibody included.

产品信息

66206-1-PBS targets Apolipoprotein AI in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen Apolipoprotein AI fusion protein Ag21920 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name apolipoprotein A-I
Synonyms APOA1, apolipoprotein A I, Apo-AI, ApoAI, ApoA I
Calculated Molecular Weight 31 kDa
Observed Molecular Weight 26-28 kDa
GenBank Accession NumberBC005380
Gene Symbol APOA1
Gene ID (NCBI) 335
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP02647
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ApoA1 is a major protein component of high density lipoproteins (HDL) which is associated with reversed cholesterol transport, lipid/cholesterol binding, lecithin/cholesterol acyltransferase (LCAT) activation and specific receptors binding. It is synthesized in the liver and small intestine. Defects of ApoA1 cause low HDL level and systemic non-neuropathic amyloidosis. Serum concentration of ApoA1 is inversely related to the risk of developing atherosclerosis. This antibody was generated against the C-terminal region of human ApoA1.