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Apolipoprotein AI Monoclonal antibody, PBS Only

Apolipoprotein AI Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C9G5

Cat No : 66206-1-PBS

Print datasheet

Synonyms

Apo AI, ApoA I, APOA1, apolipoprotein A I, Apolipoprotein A1

验证数据展示

  • WB analysis of human plasma using 66206-1-Ig (same clone as 66206-1-PBS)

    WB analysis of human plasma using 66206-1-Ig (same clone as 66206-1-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 66206-1-Ig (APOA1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66206-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66206-1-Ig (same clone as 66206-1-PBS)

    IHC staining of human liver cancer using 66206-1-Ig (same clone as 66206-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66206-1-Ig (APOA1 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66206-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with APOA1 antibody included
  • IHC staining of human liver cancer using 66206-1-Ig (same clone as 66206-1-PBS)

    IHC staining of human liver cancer using 66206-1-Ig (same clone as 66206-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66206-1-Ig (APOA1 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66206-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with APOA1 antibody included
  • IF Staining of HepG2 using 66206-1-Ig (same clone as 66206-1-PBS)

    IF Staining of HepG2 using 66206-1-Ig (same clone as 66206-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66206-1-Ig (APOA1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66206-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy APOA1 Ready-To-Use IHC Kit. APOA1 primary antibody included.

产品信息

66206-1-PBS targets Apolipoprotein AI in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF, IHC, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen Apolipoprotein AI fusion protein Ag21920 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name apolipoprotein A-I
Synonyms Apo AI, ApoA I, APOA1, apolipoprotein A I, Apolipoprotein A1
Calculated Molecular Weight 31 kDa
Observed Molecular Weight 26-28 kDa
GenBank Accession NumberBC005380
Gene Symbol APOA1
Gene ID (NCBI) 335
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP02647
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

ApoA1 is a major protein component of high density lipoproteins (HDL) which is associated with reversed cholesterol transport, lipid/cholesterol binding, lecithin/cholesterol acyltransferase (LCAT) activation and specific receptors binding. It is synthesized in the liver and small intestine. Defects of ApoA1 cause low HDL level and systemic non-neuropathic amyloidosis. Serum concentration of ApoA1 is inversely related to the risk of developing atherosclerosis. This antibody was generated against the C-terminal region of human ApoA1.