验证数据展示
经过测试的应用
Positive WB detected in | human plasma tissue |
Positive IHC detected in | human liver cancer tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
Positive FC (Intra) detected in | HepG2 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 10 publications below |
IHC | See 1 publications below |
产品信息
66206-1-Ig targets Apolipoprotein AI in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.
经测试应用 | WB, IHC, IF/ICC, FC (Intra), ELISA Application Description |
文献引用应用 | WB, IHC |
经测试反应性 | human, mouse |
文献引用反应性 | human, mouse, rat |
免疫原 | Apolipoprotein AI fusion protein Ag21920 种属同源性预测 |
宿主/亚型 | Mouse / IgG2b |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | apolipoprotein A-I |
别名 | APOA1, apolipoprotein A I, Apo-AI, ApoAI, ApoA I |
计算分子量 | 31 kDa |
观测分子量 | 26-28 kDa |
GenBank蛋白编号 | BC005380 |
基因名称 | APOA1 |
Gene ID (NCBI) | 335 |
RRID | AB_2881597 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | P02647 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ApoA1 is a major protein component of high density lipoproteins (HDL) which is associated with reversed cholesterol transport, lipid/cholesterol binding, lecithin/cholesterol acyltransferase (LCAT) activation and specific receptors binding. It is synthesized in the liver and small intestine. Defects of ApoA1 cause low HDL level and systemic non-neuropathic amyloidosis. Serum concentration of ApoA1 is inversely related to the risk of developing atherosclerosis. This antibody was generated against the C-terminal region of human ApoA1.
实验方案
Product Specific Protocols | |
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WB protocol for Apolipoprotein AI antibody 66206-1-Ig | Download protocol |
IHC protocol for Apolipoprotein AI antibody 66206-1-Ig | Download protocol |
IF protocol for Apolipoprotein AI antibody 66206-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
J Extracell Vesicles Optimized AF4 combined with density cushion ultracentrifugation enables profiling of high-purity human blood extracellular vesicles | ||
EMBO J Rab2A-mediated Golgi-lipid droplet interactions support very-low-density lipoprotein secretion in hepatocytes | ||
Mol Cell Proteomics Differential proteomic analysis of gender-dependent hepatic tumorigenesis in Hras12V transgenic mice. | ||
Peptides Neuropeptide Y promotes hepatic apolipoprotein A1 synthesis and secretion through neuropeptide Y Y5 receptor. | ||
Mol Biol Rep Differential proteomic profiles of exosomes in pediatric and adult adamantinomatous craniopharyngioma cyst fluid |