Apolipoprotein AI Monoclonal antibody

Apolipoprotein AI Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 66206-1-Ig

产品说明书

CloneNo. 1C9G5

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse and More (1)

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

APOA1, apolipoprotein A I, Apo-AI, ApoAI, ApoA I

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inhuman plasma tissue
Positive IHC detected inhuman liver cancer tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy APOA1 Ready-To-Use IHC Kit. APOA1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66206-1-Ig targets Apolipoprotein AI in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse
文献引用反应性human, mouse, rat
免疫原 Apolipoprotein AI fusion protein Ag21920 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 apolipoprotein A-I
别名 APOA1, apolipoprotein A I, Apo-AI, ApoAI, ApoA I
计算分子量 31 kDa
观测分子量 26-28 kDa
GenBank蛋白编号BC005380
基因名称 APOA1
Gene ID (NCBI) 335
RRIDAB_2881597
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP02647
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ApoA1 is a major protein component of high density lipoproteins (HDL) which is associated with reversed cholesterol transport, lipid/cholesterol binding, lecithin/cholesterol acyltransferase (LCAT) activation and specific receptors binding. It is synthesized in the liver and small intestine. Defects of ApoA1 cause low HDL level and systemic non-neuropathic amyloidosis. Serum concentration of ApoA1 is inversely related to the risk of developing atherosclerosis. This antibody was generated against the C-terminal region of human ApoA1.

实验方案

Product Specific Protocols
WB protocol for Apolipoprotein AI antibody 66206-1-IgDownload protocol
IHC protocol for Apolipoprotein AI antibody 66206-1-IgDownload protocol
IF protocol for Apolipoprotein AI antibody 66206-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Extracell Vesicles

Optimized AF4 combined with density cushion ultracentrifugation enables profiling of high-purity human blood extracellular vesicles

Authors - Liqiao Hu
mouseWB

EMBO J

Rab2A-mediated Golgi-lipid droplet interactions support very-low-density lipoprotein secretion in hepatocytes

Authors - Min Xu
humanWB

Elife

A critical role of VMP1 in lipoprotein secretion.

Authors - Hideaki Morishita
mouseWB

Mol Cell Proteomics

Differential proteomic analysis of gender-dependent hepatic tumorigenesis in Hras12V transgenic mice.

Authors - Zhuona Rong
human,ratWB

Peptides

Neuropeptide Y promotes hepatic apolipoprotein A1 synthesis and secretion through neuropeptide Y Y5 receptor.

Authors - Bingyang Liu
humanIHC

Mol Biol Rep

Differential proteomic profiles of exosomes in pediatric and adult adamantinomatous craniopharyngioma cyst fluid

Authors - Yiguang Chen
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