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验证数据展示

  • IF Staining of HepG2 using CL594-66206

    IF Staining of HepG2 using CL594-66206

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL594-66206 (Apolipoprotein AI antibody) at dilution of 1:100.

  • FC experiment of HepG2 using CL594-66206

    FC experiment of HepG2 using CL594-66206

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human Apolipoprotein AI (CL594-66206, Clone:1C9G5) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL594-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite®594-conjugated Apolipoprotein AI Monoclonal antibody

Apolipoprotein AI Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-66206

产品说明书

CloneNo. 1C9G5

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse

应用

IF/ICC, FC (Intra)

别名

APOA1, apolipoprotein A I, Apo-AI, ApoAI, ApoA I

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells, L02 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy APOA1 Ready-To-Use IHC Kit. APOA1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66206-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66206 targets Apolipoprotein AI in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse
免疫原 Apolipoprotein AI fusion protein Ag21920 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 apolipoprotein A-I
别名 APOA1, apolipoprotein A I, Apo-AI, ApoAI, ApoA I
计算分子量 31 kDa
观测分子量 26-28 kDa
GenBank蛋白编号BC005380
基因名称 APOA1
Gene ID (NCBI) 335
RRIDAB_2883535
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP02647
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ApoA1 is a major protein component of high density lipoproteins (HDL) which is associated with reversed cholesterol transport, lipid/cholesterol binding, lecithin/cholesterol acyltransferase (LCAT) activation and specific receptors binding. It is synthesized in the liver and small intestine. Defects of ApoA1 cause low HDL level and systemic non-neuropathic amyloidosis. Serum concentration of ApoA1 is inversely related to the risk of developing atherosclerosis. This antibody was generated against the C-terminal region of human ApoA1.

实验方案

Product Specific Protocols
IF protocol for CL594 Apolipoprotein AI antibody CL594-66206Download protocol
Standard Protocols
Click here to view our Standard Protocols