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Apolipoprotein E Recombinant antibody

Apolipoprotein E Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240786C3

Cat No : 83728-4-RR

Print datasheet

Synonyms

Apoe, AI255918, 240786C3

验证数据展示

  • WB analysis using 83728-4-RR

    WB analysis using 83728-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83728-4-RR (Apolipoprotein E antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 83728-4-RR

    IF Staining of HepG2 using 83728-4-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Apolipoprotein E antibody (83728-4-RR, Clone: 240786C3 ) at dilution of 1:250 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 83728-4-RR

    Affinity and Kinetic Characterization of 83728-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83728-4-RR against Mouse Apolipoprotein E were performed. The affinity constant is 0.793 nM.

经过测试的应用

Positive WB detected inmouse liver tissue, mouse serum, mouse brain tissue
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83728-4-RR is Apolipoprotein E Fusion Protein expressed in E. coli.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity mouse
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name Apolipoprotein E
Synonyms Apoe, AI255918, 240786C3
Calculated Molecular Weight36kDa
Observed Molecular Weight36 kDa
GenBank Accession NumberNM_001305819.1
Gene Symbol Apolipoprotein E
Gene ID (NCBI) 11816
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP08226
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Apolipoprotein E (apoE) is a core component of plasma lipoproteins that participates in diverse biological processes, including plasma lipoprotein metabolism, intracellular cholesterol utilization, cell growth, immunoregulation, and neuronal growth and repair (PMID: 17854398). Mouse apoE, like apoE4, contains the equivalent of Arg-112 and Glu-255, but lacks the critical Arg-61 equivalent (it contains Thr-61). Thus, mouse apoE does not display apoE4 domain interaction (PMID: 11553788).

实验方案

Product Specific Protocols
WB protocol for Apolipoprotein E antibody 83728-4-RRDownload protocol
IF protocol for Apolipoprotein E antibody 83728-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols