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CoraLite® Plus 488-conjugated Apolipoprotein H Monoclonal antibody

Apolipoprotein H Monoclonal Antibody for IF/ICC, IF-P

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

IF/ICC, IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

4D9A4

Cat no : CL488-66074

Print datasheet

Synonyms

APOH, ApolipoproteinH, Apo-H, Apo H, Activated protein C-binding protein

验证数据展示

  • IF Staining of HepG2 using CL488-66074

    IF Staining of HepG2 using CL488-66074

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 Apolipoprotein H antibody (CL488-66074, Clone: 4D9A4 ) at dilution of 1:200.

  • IF Staining of human liver cancer using CL488-66074

    IF Staining of human liver cancer using CL488-66074

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite® Plus 488 Apolipoprotein H antibody (CL488-66074, Clone: 4D9A4 ) at dilution of 1:200.

经过测试的应用

Positive IF-P detected inhuman liver cancer tissue
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy APOH Ready-To-Use IHC Kit. APOH primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66074-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66074 targets Apolipoprotein H in IF/ICC, IF-P applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications IF/ICC, IF-P Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen Apolipoprotein H fusion protein Ag17915 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name apolipoprotein H (beta-2-glycoprotein I)
Synonyms APOH, ApolipoproteinH, Apo-H, Apo H, Activated protein C-binding protein
Calculated Molecular Weight 345 aa, 38 kDa
Observed Molecular Weight 50-53 kDa
GenBank Accession NumberBC026283
Gene Symbol APOH
Gene ID (NCBI) 350
RRIDAB_2919283
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP02749
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Apolipoprotein H (ApoH), also known as beta2-glycoprotein I (B2-GPI), is a plasma glycoprotein, primarily synthesized in the liver. It has an important function in blood coagulation and clearance of apoptotic bodies from the circulation. ApoH is also an important actor of host innate immune response through its capacity to bind with high affinity to a large panel of pathogens or their proteins.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Apolipoprotein H antibody CL488-66074Download protocol
Standard Protocols
Click here to view our Standard Protocols