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验证数据展示

  • IF Staining of HepG2 using CL488-66074

    IF Staining of HepG2 using CL488-66074

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 Apolipoprotein H antibody (CL488-66074, Clone: 4D9A4 ) at dilution of 1:200.

  • IF Staining of human liver cancer using CL488-66074

    IF Staining of human liver cancer using CL488-66074

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite® Plus 488 Apolipoprotein H antibody (CL488-66074, Clone: 4D9A4 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated APOH Monoclonal antibody

APOH Monoclonal Antibody for IF/ICC, IF-P
Cat No. CL488-66074

产品说明书

CloneNo. 4D9A4

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

IF/ICC, IF-P

别名

Apolipoprotein H, ApolipoproteinH, Apo-H, Apo H, Activated protein C-binding protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inhuman liver cancer tissue
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy APOH Ready-To-Use IHC Kit. APOH primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66074-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66074 targets APOH in IF/ICC, IF-P applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 IF/ICC, IF-P Application Description
经测试反应性 human, mouse, rat, pig
免疫原 APOH fusion protein Ag17915 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 apolipoprotein H (beta-2-glycoprotein I)
别名 Apolipoprotein H, ApolipoproteinH, Apo-H, Apo H, Activated protein C-binding protein
计算分子量 345 aa, 38 kDa
观测分子量 50-53 kDa
GenBank蛋白编号BC026283
基因名称 APOH
Gene ID (NCBI) 350
RRIDAB_2919283
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP02749
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Apolipoprotein H (ApoH), also known as beta2-glycoprotein I (B2-GPI), is a plasma glycoprotein, primarily synthesized in the liver. It has an important function in blood coagulation and clearance of apoptotic bodies from the circulation. ApoH is also an important actor of host innate immune response through its capacity to bind with high affinity to a large panel of pathogens or their proteins.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 APOH antibody CL488-66074Download protocol
Standard Protocols
Click here to view our Standard Protocols