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验证数据展示

  • WB analysis using 67326-1-Ig

    WB analysis using 67326-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67326-1-Ig (Arp5/ACTR5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 67326-1-Ig

    WB analysis of HEK-293 using 67326-1-Ig

    WB result of Arp5/ACTR5 antibody (67326-1-Ig; 1:80000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Arp5/ACTR5 transfected HEK-293 cells.

  • Featured Product
  • KD/KO验证

Arp5/ACTR5 Monoclonal antibody

Arp5/ACTR5 Monoclonal Antibody for WB, ELISA
Cat No. 67326-1-Ig

产品说明书

CloneNo. 1E11C1

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat, pig

应用

WB, ELISA

别名

Actin related protein 5, ACTR5, Arp5, Arp5/ACTR5, FLJ12785, hARP5, INO80M, Sarcoma antigen NY SAR 16

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, C2C12 cells, pig skeletal muscle tissue, rat skeletal muscle tissue, HEK-293 cells, K-562 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67326-1-Ig targets Arp5/ACTR5 in WB, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human, mouse, rat, pig
免疫原 Arp5/ACTR5 fusion protein Ag15976 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ARP5 actin-related protein 5 homolog (yeast)
别名 Actin related protein 5, ACTR5, Arp5, Arp5/ACTR5, FLJ12785, hARP5, INO80M, Sarcoma antigen NY SAR 16
计算分子量 607 aa, 68 kDa
观测分子量 68 kDa
GenBank蛋白编号BC038402
基因名称 ARP5/ACTR5
Gene ID (NCBI) 79913
RRIDAB_2882585
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9H9F9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Arp5 is a member of nuclear actin-related proteins (ARPs) that function as components of the chromatin- remodeling complex. Recently study reported that Arp5 regulates smooth muscle cell differentiation through interaction with myocardin (Myocd). Arp5 has been identified as negative regulator of Myocd, suppressing Myocd activity in the nucleus through tightly binding with Myocd RPEL motifs. Alternative splicing results in two variants of Arp5: a broadly expressed arp5-common, and a smooth muscle restricted arp5-sm. Expression of Arp5-sm protein is strongly suppressed in well-differentiated smooth muscle cells by partial messenger RNA decay and translational suppression. (24567363)

实验方案

Product Specific Protocols
WB protocol for Arp5/ACTR5 antibody 67326-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols