验证数据展示
经过测试的应用
Positive IF-P detected in | mouse brain tissue |
推荐稀释比
应用 | 推荐稀释比 |
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Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-21776 targets Ataxin 2 in IF-P applications and shows reactivity with human, mouse, rat samples.
经测试应用 | IF-P Application Description |
经测试反应性 | human, mouse, rat |
免疫原 | Ataxin 2 fusion protein Ag16470 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | ataxin 2 |
别名 | ataxin 2, ATX2, ATXN2, FLJ46772, SCA2, TNRC13 |
计算分子量 | 1313 aa, 140 kDa |
观测分子量 | 140-150 kDa |
GenBank蛋白编号 | BC114546 |
基因名称 | ATXN2 |
Gene ID (NCBI) | 6311 |
RRID | AB_2934415 |
偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
最大激发/发射波长 | 493 nm / 522 nm |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q99700 |
储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Background
ATXN2 (Ataxin-2) is a eukaryotic RNA‐binding protein that is conserved across species. It contains two LSm domains (RNA binding), a PAM2 motif (association with poly(A)-binding protein), and an N-terminal polyglutamine tract (PMID: 25027299). Ataxin-2 plays crucial roles at different stages of the regulation of posttranslational gene expression. ATXN2 is also directly implicated in the regulation of neural function by influencing specific molecular and cellular pathways. Genetic expansion of a poly‐glutamine tract in human ATXN2 has been linked to several neurodegenerative diseases, where it most likely acts through gain‐of‐function effects.
What is the molecular weight of ATXN2?
The molecular weight varies from 12 to 140 kDa, depending on the isoform.
What are the isoforms of ATXN2?
ATXN2 has many isoforms, which differ significantly in length and domain composition.
What is the subcellular localization of ATXN2?
According to siRNA-based experiments, it is localized in the cytoplasmic compartment of normal cells, where it mostly associates with the Golgi apparatus and stress granules (PMID: 22508507).
What is the tissue specificity of ATXN2?
ATXN2 protein has been detected in various tissues; however, it is most highly expressed and studied in CNS. Significant amounts of this protein can also be detected in the liver and gallbladder.
What is the molecular function of ATXN2?
Ataxin-2 is involved in regulating various steps of mRNA translation, including poly‐A tailing, RNA stabilization, microRNA‐dependent gene silencing, and translational activation. All those functions are linked to its interactions with the poly(A)-binding protein. Ataxin-2 is involved in the formation of stress granules and P-bodies. Furthermore, genetic models of ATXN2 loss‐of‐function have underlined the importance of ATXN2 in mTOR signaling and cellular metabolism, which are all crucial for neural homeostasis (PMID: 29869836).
What is ATXN2's involvement in disease?
The N-terminal region of ATXN2 normally contains a polyQ stretch of 14-31 residues that can be expanded in the pathogenic state to 32-200 residues. Intermediate length expansions of this tract increase susceptibility to amyotrophic lateral sclerosis (ALS or Lou Gehrig's disease) (PMID: 20740007). Long expansions of this tract result in spinocerebellar ataxia-2 (SCA2), an autosomal dominantly inherited, neurodegenerative disorder (PMID: 29427103). Genome-wide association studies indicate that loss-of-function mutations in this gene are associated with susceptibility to type I diabetes, obesity, and hypertension.
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 Ataxin 2 antibody CL488-21776 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |