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Mouse Atox1 Recombinant antibody, PBS Only (Capture)

Atox1 Uni-rAbTM Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241566A7

Cat no : 84305-1-PBS

Print datasheet

Synonyms

HAH1, Atx1, 241566A7

验证数据展示

  • WB analysis using 84305-1-RR (same clone as 84305-1-PBS)

    WB analysis using 84305-1-RR (same clone as 84305-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84305-1-RR (Atox1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84305-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 84305-1-RR (same clone as 84305-1-PBS)

    IHC staining of mouse heart using 84305-1-RR (same clone as 84305-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 84305-1-RR (Atox1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84305-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01193-1

    Cytometric bead array standard curve of MP01193-1

    Cytometric bead array standard curve of MP01193-1, MOUSE Atox1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84305-1-PBS. Detection antibody: 84305-2-PBS. Standard: Ag35290. Range: 0.313-40 ng/mL.

产品信息

84305-1-PBS targets Atox1 as part of a matched antibody pair:

MP01193-1: 84305-1-PBS capture and 84305-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen Atox1 fusion protein Ag35290 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ATX1 (antioxidant protein 1) homolog 1 (yeast)
Synonyms HAH1, Atx1, 241566A7
Calculated Molecular Weight7 kDa
Observed Molecular Weight7 kDa
GenBank Accession NumberNM_009720
Gene Symbol Atox1
Gene ID (NCBI) 11927
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO08997
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Antioxidant 1 copper chaperone (Atox1) is a small cytosolic protein containing an MBS (metal binding site) and a potential NLS (nuclear localisation signal). It plays an essential role in copper homeostasis by facilitating the transfer of copper to the trans-Golgi. Atox1 has been implicated in a wide range of diseases, including many neurodegenerative, cancer and metabolic disorders.