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验证数据展示

  • FC experiment of K-562 using CL488-66711

    FC experiment of K-562 using CL488-66711

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human CD57 (CL488-66711, Clone:1F4A11) (red), or 0.4 ug CoraLite® Plus 488 Mouse IgG2a Isotype Control (C1.18.4) (CL488-65208, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated B3GAT1 Monoclonal antibody

B3GAT1 Monoclonal Antibody for FC (Intra)
Cat No. CL488-66711

产品说明书

CloneNo. 1F4A11

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

FC (Intra)

别名

GlcAT-P, GLCATP, Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1, EC:2.4.1.135, Beta-1,3-glucuronyltransferase 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inK-562 cells
For other applications, we recommend the unconjugated version of this antibody, 66711-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66711 targets B3GAT1 in FC (Intra) applications and shows reactivity with human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human
免疫原 B3GAT1 fusion protein Ag13593 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P)
别名 GlcAT-P, GLCATP, Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1, EC:2.4.1.135, Beta-1,3-glucuronyltransferase 1
计算分子量 38 kDa
观测分子量 38 kDa, 100 kDa
GenBank蛋白编号BC010466
基因名称 B3GAT1
Gene ID (NCBI) 27087
RRIDAB_3084267
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9P2W7
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

B3GAT1 is an enzyme encoded by the B3GAT1 gene, whose enzymatic activity generates the CD57 epitope on other cell surface proteins. In immunology, the CD57 antigen (CD stands for cluster of differentiation) is also known as HNK1 (human natural killer-1) or LEU7. B3GAT1 is associated with glycosaminoglycan biosynthesis. It plays a key role in neurodevelopment.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 B3GAT1 antibody CL488-66711Download protocol
Standard Protocols
Click here to view our Standard Protocols