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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated B7-H3/CD276 Monoclonal antibody

B7-H3/CD276 Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Pig

Applications

IF-P

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1E7D1

Cat no : CL594-66481

Print datasheet

Synonyms

4Ig B7 H3, B7 H3, B7 homolog 3, B7H3, B7-H3, CD276, CD276 antigen, CD276 molecule, Costimulatory molecule, UNQ309/PRO352

验证数据展示

  • IF Staining of human tonsillitis using CL594-66481

    IF Staining of human tonsillitis using CL594-66481

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594-conjugated B7-H3/CD276 antibody (CL594-66481, Clone: 1E7D1 ) at dilution of 1:400.

经过测试的应用

Positive IF-P detected inhuman tonsillitis tissue
For other applications, we recommend the unconjugated version of this antibody, 66481-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66481 targets B7-H3/CD276 in IF-P applications and shows reactivity with Human, Pig samples.

Tested Applications IF-P Application Description
Tested Reactivity Human, Pig
Immunogen B7-H3/CD276 fusion protein Ag6006 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name CD276 molecule
Synonyms 4Ig B7 H3, B7 H3, B7 homolog 3, B7H3, B7-H3, CD276, CD276 antigen, CD276 molecule, Costimulatory molecule, UNQ309/PRO352
Calculated Molecular Weight 57 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC062581
Gene Symbol CD276
Gene ID (NCBI) 80381
RRIDAB_2883588
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ5ZPR3
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

B7-H3 (CD276) is a type I transmembrane protein expressed on many tissues and cell types. B7-H3 is a 100-kDa glycoprotein that belongs to the B7 immunoregulatory family and participates in the regulation of T-cell-mediated immune response probably by functioning as both a T cell costimulator and coinhibitor (PMID: 25567370; 20696859). Overexpressed on a wide range of human solid cancers, B7-H3 has been implicated in cancer progression and metastasis and becomes an attractive target for cancer immunotherapy (PMID: 27208063).

实验方案

Product Specific Protocols
IF protocol for CL594 B7-H3/CD276 antibody CL594-66481Download protocol
Standard Protocols
Click here to view our Standard Protocols