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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated BAG1 Polyclonal antibody

BAG1 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-19064

Print datasheet

Synonyms

BAG 1, BAG1, BAG1L speicfic, BAG1M, BAG1S, HAP, RAP46

验证数据展示

  • IF Staining of HeLa using CL488-19064

    IF Staining of HeLa using CL488-19064

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 BAG1 antibody (CL488-19064) at dilution of 1:200, CL594-Phalloidin (red).

经过测试的应用

Positive IF/ICC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 19064-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-19064 targets BAG1 in IF/ICC applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse
Immunogen BAG1 fusion protein Ag13555 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name BCL2-associated athanogene
Synonyms BAG 1, BAG1, BAG1L speicfic, BAG1M, BAG1S, HAP, RAP46
Calculated Molecular Weight 39 kDa
Observed Molecular Weight 50-55 kDa, 30-33 kDa
GenBank Accession NumberBC014774
Gene Symbol BAG1
Gene ID (NCBI) 573
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99933
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BAG1 have been identified that modulate gene transcription through poorly defined mechanisms. Four isoforms of the BAG1 protein (BAG1S, BAG1, BAG1M and BAG1L) can be produced from a common mRNA by use of alternative translation initiation sites, including a non-canonical CTG codon in one instance. The longest, BAG1L (Mr ~50K), contains a nuclear localization signal (NLS) and resides in the nucleus, whereas BAG1M (Mr ~46K) has an incomplete NLS and distributes mainly in cytosol, unless dragged into the nucleus through interactions with other. Distribution of BAG1S(p33) is not clear yet. This antibody can recognize all the isoforms of BAG1.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 BAG1 antibody CL488-19064Download protocol
Standard Protocols
Click here to view our Standard Protocols