验证数据展示
经过测试的应用
| Positive WB detected in | NIH/3T3 cells, LNCaP cells, rabbit heart tissue, HeLa cells, pig heart, rat heart |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
68076-1-Ig targets BAG3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat, pig, rabbit |
| 文献引用反应性 | human, rat |
| 免疫原 | BAG3 fusion protein Ag17608 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | BCL2-associated athanogene 3 |
| 别名 | Docking protein CAIR-1, BIS, Bcl-2-binding protein Bis, Bcl-2-associated athanogene 3, BAG-3 |
| 计算分子量 | 61 kDa |
| 观测分子量 | 74 kDa |
| GenBank蛋白编号 | BC006418 |
| 基因名称 | BAG3 |
| Gene ID (NCBI) | 9531 |
| RRID | AB_2918814 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | O95817 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
BAG3 (Bcl2-associated athanogene 3) belongs to the BAG protein family, the co-chaperone that binds to Hsc70/Hsp70 through the BAG domain and modulates their activity in polypeptide folding. BAG3 contains also a WW domain and a proline-rich (PXXP) repeat, that mediate binding to partners different from Hsp70. Through interacting with different molecular partner, BAG3 influences several cell processes, such as apoptosis, autophagy and cell motility. BAG3 protein has been reported to sustain cell survival, resistance to therapy, and/or motility and metastatization in several tumor types, thus being identified as a potential target for anticancer therapies. In addition, defects in BAG3 are the cause of some myopathy.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for BAG3 antibody 68076-1-Ig | Download protocol |
| IF protocol for BAG3 antibody 68076-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Physiol Biochem BAG3 protects chondrocytes against lumbar facet joint osteoarthritis by regulating autophagy and apoptosis. | ||
J Physiol Sci High-intensity interval training alleviates exhaustive exercise-induced HSP70-assisted selective autophagy in skeletal muscle |
