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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated BAG3 Polyclonal antibody

BAG3 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

Cat No : CL594-10599

Print datasheet

Synonyms

BAG 3, BAG3, BIS, CAIR 1, Docking protein CAIR 1

验证数据展示

  • IF Staining of A549 using CL594-10599

    IF Staining of A549 using CL594-10599

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using CoraLite®594 BAG3 antibody (CL594-10599) at dilution of 1:100.

  • IF Staining of A549 using CL594-10599

    IF Staining of A549 using CL594-10599

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using CoraLite®594 BAG3 antibody (CL594-10599) at dilution of 1:200.

经过测试的应用

Positive IF detected inA549 cells
Planning an IHC experiment? We recommend our IHCeasy BAG3 Ready-To-Use IHC Kit. BAG3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 10599-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-10599 targets BAG3 in IF applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC
Tested Reactivity human, mouse, rat
Immunogen BAG3 fusion protein Ag0956 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name BCL2-associated athanogene 3
Synonyms BAG 3, BAG3, BIS, CAIR 1, Docking protein CAIR 1
Calculated Molecular Weight 61 kDa
Observed Molecular Weight 74-80 kDa
GenBank Accession NumberBC006418
Gene Symbol BAG3
Gene ID (NCBI) 9531
RRIDAB_2919741
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95817
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BAG3 (Bcl2-associated athanogene 3) belongs to the BAG protein family, the co-chaperone that binds to Hsc70/Hsp70 through the BAG domain and modulates their activity in polypeptide folding. BAG3 contains also a WW domain and a proline-rich (PXXP) repeat, that mediate binding to partners different from Hsp70. Through interacting with different molecular partner, BAG3 influences several cell processes, such as apoptosis, autophagy and cell motility. BAG3 protein has been reported to sustain cell survival, resistance to therapy, and/or motility and metastatization in several tumor types, thus being identified as a potential target for anticancer therapies. In addition, defects in BAG3 are the cause of some myopathy. BAG3 normally migrates around 74-80 kDa; a slightly different molecular weight or a doublet form can be observed in some cell types and/or following cell exposure to stressors. A synaptosome associated form of 40 kDa has recently been described.

实验方案

Product Specific Protocols
IF protocol for CL594 BAG3 antibody CL594-10599Download protocol
Standard Protocols
Click here to view our Standard Protocols