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验证数据展示

  • IF Staining of A549 using CL594-10599

    IF Staining of A549 using CL594-10599

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using CoraLite®594 BAG3 antibody (CL594-10599) at dilution of 1:100.

  • IF Staining of A549 using CL594-10599

    IF Staining of A549 using CL594-10599

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using CoraLite®594 BAG3 antibody (CL594-10599) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite®594-conjugated BAG3 Polyclonal antibody

BAG3 Polyclonal Antibody for IF/ICC
Cat No. CL594-10599

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC

别名

MFM6, Docking protein CAIR-1, BIS, Bcl-2-binding protein Bis, Bcl-2-associated athanogene 3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inA549 cells
Planning an IHC experiment? We recommend our IHCeasy BAG3 Ready-To-Use IHC Kit. BAG3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 10599-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-10599 targets BAG3 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat
免疫原 BAG3 fusion protein Ag0956 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 BCL2-associated athanogene 3
别名 MFM6, Docking protein CAIR-1, BIS, Bcl-2-binding protein Bis, Bcl-2-associated athanogene 3
计算分子量 61 kDa
观测分子量 74-80 kDa
GenBank蛋白编号BC006418
基因名称 BAG3
Gene ID (NCBI) 9531
RRIDAB_2919741
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95817
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BAG3 (Bcl2-associated athanogene 3) belongs to the BAG protein family, the co-chaperone that binds to Hsc70/Hsp70 through the BAG domain and modulates their activity in polypeptide folding. BAG3 contains also a WW domain and a proline-rich (PXXP) repeat, that mediate binding to partners different from Hsp70. Through interacting with different molecular partner, BAG3 influences several cell processes, such as apoptosis, autophagy and cell motility. BAG3 protein has been reported to sustain cell survival, resistance to therapy, and/or motility and metastatization in several tumor types, thus being identified as a potential target for anticancer therapies. In addition, defects in BAG3 are the cause of some myopathy. BAG3 normally migrates around 74-80 kDa; a slightly different molecular weight or a doublet form can be observed in some cell types and/or following cell exposure to stressors. A synaptosome associated form of 40 kDa has recently been described.

实验方案

Product Specific Protocols
IF protocol for CL594 BAG3 antibody CL594-10599Download protocol
Standard Protocols
Click here to view our Standard Protocols