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BAG6 Polyclonal antibody
BAG6 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IHC, IF/ICC, IP, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | A431 cells, HEK-293 cells, HeLa cells |
Positive IP detected in | A431 cells |
Positive IHC detected in | human breast cancer tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:16000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
The immunogen of 26417-1-AP is BAG6 Fusion Protein expressed in E. coli.
Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
Cited Applications | WB, IP, IF, IHC, CoIP |
Tested Reactivity | human |
Cited Reactivity | human, mouse |
Immunogen | BAG6 fusion protein Ag24525 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | HLA-B associated transcript 3 |
Synonyms | BAG 6, BAG6, BAT3, D6S52E, G3, HLA B associated transcript 3, Protein G3 |
Calculated Molecular Weight | 119 kDa |
Observed Molecular Weight | 150 kDa |
GenBank Accession Number | BC003133 |
Gene Symbol | BAT3/BAG-6 |
Gene ID (NCBI) | 7917 |
RRID | AB_2880508 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P46379 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for BAG6 antibody 26417-1-AP | Download protocol |
IHC protocol for BAG6 antibody 26417-1-AP | Download protocol |
IF protocol for BAG6 antibody 26417-1-AP | Download protocol |
IP protocol for BAG6 antibody 26417-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Clin Transl Med Deubiquitinase USP19 modulates apoptotic calcium release and endoplasmic reticulum stress by deubiquitinating BAG6 in triple negative breast cancer | ||
EMBO Rep UBXN1 maintains ER proteostasis and represses UPR activation by modulating translation | ||
Mol Cell Biol The VCP-UBXN1 complex mediates triage of ubiquitylated cytosolic proteins bound to the BAG6 complex.
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Exp Eye Res Retinitis pigmentosa 2 pathogenic mutants degrade through BAG6/HUWE1 complex.
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Front Immunol BAG6 negatively regulates the RLR signaling pathway by targeting VISA/MAVS
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