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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated BAG6 Polyclonal antibody

BAG6 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-26417

Print datasheet

Synonyms

BAG 6, BAG6, BAT3, D6S52E, G3, HLA B associated transcript 3, Protein G3

验证数据展示

  • IF Staining of HeLa using CL488-26417

    IF Staining of HeLa using CL488-26417

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 BAG6 antibody (CL488-26417) at dilution of 1:200.

  • FC experiment of HeLa using CL488-26417

    FC experiment of HeLa using CL488-26417

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human BAG6 (CL488-26417)(red), or 0.8 ug Control Antibody (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 26417-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-26417 targets BAG6 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human
Immunogen BAG6 fusion protein Ag24525 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name HLA-B associated transcript 3
Synonyms BAG 6, BAG6, BAT3, D6S52E, G3, HLA B associated transcript 3, Protein G3
Calculated Molecular Weight 119 kDa
Observed Molecular Weight 150 kDa
GenBank Accession NumberBC003133
Gene Symbol BAT3/BAG-6
Gene ID (NCBI) 7917
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP46379
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BAT3 also known as Scythe or BAG6, is a nuclear protein implicated in the control of apoptosis and natural killer (NK) cell-dendritic cell (DC) interaction. BAT3 was first discovered as a member of a group of genes located within the class III region of the human major histocompatibility complex on chromosome 6, and has been extensively studied for its role in regulating apoptosis under various stress conditions such as DNA damage and endoplasmic reticulum-related stress. BAT3 has been shown to be required for p53 acetylation, which is critical for the enhancement of p53 transcriptional activity in response to DNA damage. In addition, BAT3 is involved in the regulation of development and reproduction of mammals by acting as a co-chaperone of the heat shock protein HSP70.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 BAG6 antibody CL488-26417Download protocol
FC protocol for CL Plus 488 BAG6 antibody CL488-26417Download protocol
Standard Protocols
Click here to view our Standard Protocols