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BBS10 Monoclonal antibody

BBS10 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

4C6A10

Cat no : 66679-1-Ig

Print datasheet

Synonyms

Bardet Biedl syndrome 10, BBS10, C12orf58, FLJ23560

验证数据展示

  • WB analysis using 66679-1-Ig

    WB analysis using 66679-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66679-1-Ig (BBS10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • IHC staining of human liver cancer using 66679-1-Ig

    IHC staining of human liver cancer using 66679-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66679-1-Ig (BBS10 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 66679-1-Ig

    WB analysis using 66679-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66679-1-Ig (BBS10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inNCI-H1299 cells, NCCIT cells, L02 cells, HepG2 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66679-1-Ig targets BBS10 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human
Immunogen BBS10 fusion protein Ag17960 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name Bardet-Biedl syndrome 10
Synonyms Bardet Biedl syndrome 10, BBS10, C12orf58, FLJ23560
Calculated Molecular Weight 81 kDa
Observed Molecular Weight 81 kDa
GenBank Accession NumberBC013795
Gene Symbol BBS10
Gene ID (NCBI) 79738
RRIDAB_2882033
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8TAM1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BBS10, also named as C12orf58, belongs to the TCP-1 chaperonin family. It is probable molecular chaperone. Assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex, BBS10 may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Involved in adipogenic differentiation. (PMID: 20080638, 19190184) Defects in BBS10 are the cause of Bardet-Biedl syndrome type 10 (BBS10).

实验方案

Product Specific Protocols
WB protocol for BBS10 antibody 66679-1-IgDownload protocol
IHC protocol for BBS10 antibody 66679-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols