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BBS10 Monoclonal antibody, PBS Only

BBS10 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4C6A10

Cat no : 66679-1-PBS

Print datasheet

Synonyms

Bardet Biedl syndrome 10, BBS10, C12orf58, FLJ23560

验证数据展示

  • WB analysis using 66679-1-Ig (same clone as 66679-1-PBS)

    WB analysis using 66679-1-Ig (same clone as 66679-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66679-1-Ig (BBS10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66679-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66679-1-Ig (same clone as 66679-1-PBS)

    IHC staining of human liver cancer using 66679-1-Ig (same clone as 66679-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66679-1-Ig (BBS10 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66679-1-PBS in a different storage buffer formulation.

  • WB analysis using 66679-1-Ig (same clone as 66679-1-PBS)

    WB analysis using 66679-1-Ig (same clone as 66679-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66679-1-Ig (BBS10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66679-1-PBS in a different storage buffer formulation.

产品信息

66679-1-PBS targets BBS10 in WB, IHC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity Human
Immunogen BBS10 fusion protein Ag17960 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name Bardet-Biedl syndrome 10
Synonyms Bardet Biedl syndrome 10, BBS10, C12orf58, FLJ23560
Calculated Molecular Weight 81 kDa
Observed Molecular Weight 81 kDa
GenBank Accession NumberBC013795
Gene Symbol BBS10
Gene ID (NCBI) 79738
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8TAM1
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

BBS10, also named as C12orf58, belongs to the TCP-1 chaperonin family. It is probable molecular chaperone. Assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex, BBS10 may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Involved in adipogenic differentiation. (PMID: 20080638, 19190184) Defects in BBS10 are the cause of Bardet-Biedl syndrome type 10 (BBS10).