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BCAM Polyclonal antibody

BCAM Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat No : 26528-1-AP

Print datasheet

Synonyms

AU, Auberger B antigen, Basal cell adhesion molecule, BCAM, CD239, F8/G253 antigen, LU, Lutheran antigen, MSK19

验证数据展示

  • WB analysis using 26528-1-AP

    WB analysis using 26528-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26528-1-AP (BCAM antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inA431 cells, HT-29 cells, human placenta tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26528-1-AP targets BCAM in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen BCAM fusion protein Ag24753 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name basal cell adhesion molecule (Lutheran blood group)
Synonyms AU, Auberger B antigen, Basal cell adhesion molecule, BCAM, CD239, F8/G253 antigen, LU, Lutheran antigen, MSK19
Calculated Molecular Weight 67 kDa
Observed Molecular Weight78 kDa, 85 kDa
GenBank Accession NumberBC050450
Gene Symbol BCAM
Gene ID (NCBI) 4059
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP50895
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Basal cell adhesion molecule (Lu/BCAM) is a 90 kDa membrane-bound glycoprotein of the immunoglobulin superfamily (IgSF), functioning as a receptor for the extracellular matrix protein, laminin. BCAM, involved in cell adhesion and migration, can also promote tumor metastasis and has been elucidated to play a functional role in the metastasis of thyroid cancer and gastric cancer (PMID: 35941663). Two Lu/BCAM glycoprotein (gp) isoforms are present on human RBCs, Lu (85 kDa) and Lu(v13) (78 kDa); they result from the alternative splicing of intron 13 and differ by the lengths of their cytoplasmic domains (PMID: 23160466)..

实验方案

Product Specific Protocols
WB protocol for BCAM antibody 26528-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols