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BCAM Polyclonal antibody

BCAM Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat No : 28222-1-AP

Print datasheet

Synonyms

AU, Auberger B antigen, Basal cell adhesion molecule, BCAM, CD239, F8/G253 antigen, LU, Lutheran antigen, MSK19

验证数据展示

  • WB analysis using 28222-1-AP

    WB analysis using 28222-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28222-1-AP (BCAM antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inA431 cells, HT-29 cells, human placenta tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28222-1-AP targets BCAM in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen BCAM fusion protein Ag28181 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name basal cell adhesion molecule (Lutheran blood group)
Synonyms AU, Auberger B antigen, Basal cell adhesion molecule, BCAM, CD239, F8/G253 antigen, LU, Lutheran antigen, MSK19
Calculated Molecular Weight 67 kDa
Observed Molecular Weight67-80 kDa
GenBank Accession NumberBC050450
Gene Symbol BCAM
Gene ID (NCBI) 4059
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP50895
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BCAM (Lutheran/basal cell-adhesion molecule, a glycoprotein) belongs to the immunoglobulin superfamily which contains both Lu blood group and BCAM tumor-associated antigens. The significance of Lu/BCAM on tumorigenesis has been increasingly recognized. Lu/BCAM is involved in cell-cell adhesion and migration in skin tumor (PMID: 11121140, PMID: 10728810). Proteomics analysis suggests that BCAM might be a potential biomarker for pancreatic cancer (PMID: 19199705).

实验方案

Product Specific Protocols
WB protocol for BCAM antibody 28222-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols