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验证数据展示

  • WB analysis using 84038-3-RR

    WB analysis using 84038-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84038-3-RR (BCAM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84038-3-RR

    Affinity and Kinetic Characterization of 84038-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84038-3-RR against Human BCAM were performed. The affinity constant is 6.16 nM.

BCAM Recombinant antibody

BCAM Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 84038-3-RR

Print datasheet

CloneNo. 241051C8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

F8/G253 antigen, CD239, B-CAM cell surface glycoprotein, Basal cell adhesion molecule, Auberger B antigen

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, T-47D cells, human placenta tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84038-3-RR targets BCAM in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen BCAM fusion protein Ag28181 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name basal cell adhesion molecule (Lutheran blood group)
Synonyms F8/G253 antigen, CD239, B-CAM cell surface glycoprotein, Basal cell adhesion molecule, Auberger B antigen
Calculated Molecular Weight 67 kDa
Observed Molecular Weight67 kDa, 78-85 kDa
GenBank Accession NumberBC050450
Gene Symbol BCAM
Gene ID (NCBI) 4059
RRIDAB_3671605
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP50895
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Basal cell adhesion molecule (Lu/BCAM) is a membrane-bound glycoprotein of the immunoglobulin superfamily (IgSF), functioning as a receptor for the extracellular matrix protein, laminin. BCAM (Lutheran/basal cell-adhesion molecule, a glycoprotein) belongs to the immunoglobulin superfamily which contains both Lu blood group and BCAM tumor-associated antigens. Proteomics analysis suggests that BCAM might be a potential biomarker for pancreatic cancer (PMID: 19199705). BCAM, involved in cell adhesion and migration, can also promote tumor metastasis and has been elucidated to play a functional role in the metastasis of thyroid cancer and gastric cancer (PMID: 10728810, 35941663). De-glycosylated BCAM has a preidicted molecular weight of 67 kDa. Two BCAM glycoprotein (gp) isoforms are present on human RBCs, Lu (85 kDa) and Lu(v13) (78 kDa); they result from the alternative splicing of intron 13 and differ by the lengths of their cytoplasmic domains (PMID: 23160466).

实验方案

Product Specific Protocols
WB protocol for BCAM antibody 84038-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols