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BCAT1 Polyclonal antibody, PBS Only

BCAT1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 28622-1-PBS

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Synonyms

验证数据展示

  • WB analysis using 28622-1-AP (same clone as 28622-1-PBS)

    WB analysis using 28622-1-AP (same clone as 28622-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28622-1-AP (BCAT1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28622-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 28622-1-AP (same clone as 28622-1-PBS)

    IHC staining of human liver cancer using 28622-1-AP (same clone as 28622-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28622-1-AP (BCAT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28622-1-PBS in a different storage buffer formulation.

产品信息

28622-1-PBS targets BCAT1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human, rat
Immunogen BCAT1 fusion protein Ag28998 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name branched chain aminotransferase 1, cytosolic
Synonyms
Calculated Molecular Weight 320 aa, 36 kDa
Observed Molecular Weight 43-48 kDa
GenBank Accession NumberBC033864
Gene Symbol BCAT1
Gene ID (NCBI) 586
RRIDAB_2881183
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP54687
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

BCAT1, also named as branched-chain amino acid trasaminase1, is a cytosolic enzyme responsible for the reversible transamination of leucine, isoleucine and valine that catalyzes the transformation of branched-chain L-amino acids (BCAA) into branched-chain a-ketoacids (BCKA), thereby providing macromolecule precursors. It has been reported that BCAT1 is associated with tumor growth and disease progression (PMID:29255149).