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BCAT1 Monoclonal antibody

BCAT1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1G3A10

Cat no : 67084-1-Ig

Print datasheet

Synonyms

BCAT1, DKFZp686E12175, ECA39, MECA39, PNAS 121, PP18, Protein ECA39

验证数据展示

  • WB analysis of Ramos using 67084-1-Ig

    WB analysis of Ramos using 67084-1-Ig

    Ramos cells were subjected to SDS PAGE followed by western blot with 67084-1-Ig (BCAT1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inRamos cells, Daudi cells, pig brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67084-1-Ig targets BCAT1 in WB, ELISA applications and shows reactivity with Human, pig samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, pig
Immunogen BCAT1 fusion protein Ag28328 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name branched chain aminotransferase 1, cytosolic
Synonyms BCAT1, DKFZp686E12175, ECA39, MECA39, PNAS 121, PP18, Protein ECA39
Calculated Molecular Weight 320 aa, 36 kDa
Observed Molecular Weight 42-45 kDa
GenBank Accession NumberBC033864
Gene Symbol BCAT1
Gene ID (NCBI) 586
RRIDAB_2882392
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP54687
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

BCAT1, also named as branched-chain amino acid trasaminase1, is a cytosolic enzyme responsible for the reversible transamination of leucine, isoleucine and valine that catalyzes the transformation of branched-chain L-amino acids (BCAA) into branched-chain a-ketoacids (BCKA), thereby providing macromolecule precursors. It has been reported that BCAT1 is associated with tumor growth and disease progression (PMID:29255149).

实验方案

Product Specific Protocols
WB protocol for BCAT1 antibody 67084-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols