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CoraLite® Plus 488-conjugated BCAT1 Monoclonal antibody

BCAT1 Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1G3A10

Cat no : CL488-67084

Print datasheet

Synonyms

MECA39, ECA39, EC:2.6.1.42, Branched-chain-amino-acid aminotransferase, cytosolic, BCAT1/ECA39

验证数据展示

  • WB analysis using CL488-67084

    WB analysis using CL488-67084

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-67084 (BCAT1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inDaudi cells
For other applications, we recommend the unconjugated version of this antibody, 67084-1-Ig

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67084 targets BCAT1 in WB applications and shows reactivity with human, pig samples.

Tested Applications WB Application Description
Tested Reactivity human, pig
Immunogen BCAT1 fusion protein Ag28328 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name branched chain aminotransferase 1, cytosolic
Synonyms MECA39, ECA39, EC:2.6.1.42, Branched-chain-amino-acid aminotransferase, cytosolic, BCAT1/ECA39
Calculated Molecular Weight 320 aa, 36 kDa
Observed Molecular Weight 42-45 kDa
GenBank Accession NumberBC033864
Gene Symbol BCAT1
Gene ID (NCBI) 586
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP54687
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BCAT1, also named as branched-chain amino acid trasaminase1, is a cytosolic enzyme responsible for the reversible transamination of leucine, isoleucine and valine that catalyzes the transformation of branched-chain L-amino acids (BCAA) into branched-chain a-ketoacids (BCKA), thereby providing macromolecule precursors. It has been reported that BCAT1 is associated with tumor growth and disease progression (PMID:29255149).

实验方案

Product Specific Protocols
WB protocol for CL Plus 488 BCAT1 antibody CL488-67084Download protocol
Standard Protocols
Click here to view our Standard Protocols