验证数据展示
经过测试的应用
| Positive WB detected in | Daudi cells |
For other applications, we recommend the unconjugated version of this antibody, 67084-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-67084 targets BCAT1 in WB applications and shows reactivity with human, pig samples.
| 经测试应用 | WB Application Description |
| 经测试反应性 | human, pig |
| 免疫原 | BCAT1 fusion protein Ag28328 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | branched chain aminotransferase 1, cytosolic |
| 别名 | MECA39, ECA39, EC:2.6.1.42, Branched-chain-amino-acid aminotransferase, cytosolic, BCAT1/ECA39 |
| 计算分子量 | 320 aa, 36 kDa |
| 观测分子量 | 42-45 kDa |
| GenBank蛋白编号 | BC033864 |
| 基因名称 | BCAT1 |
| Gene ID (NCBI) | 586 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P54687 |
| 储存缓冲液 | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
BCAT1, also named as branched-chain amino acid trasaminase1, is a cytosolic enzyme responsible for the reversible transamination of leucine, isoleucine and valine that catalyzes the transformation of branched-chain L-amino acids (BCAA) into branched-chain a-ketoacids (BCKA), thereby providing macromolecule precursors. It has been reported that BCAT1 is associated with tumor growth and disease progression (PMID:29255149).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CL Plus 488 BCAT1 antibody CL488-67084 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |