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  • Featured Product
  • KD/KO Validated

BCL10 Polyclonal antibody

BCL10 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 17732-1-AP

Print datasheet

Synonyms

CARD-containing molecule enhancing NF-kappa-B, c E10, Bcl-10, Bcl 10, B-cell lymphoma/leukemia 10

验证数据展示

  • WB analysis using 17732-1-AP

    WB analysis using 17732-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17732-1-AP (BCL10 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of K-562 using 17732-1-AP

    WB analysis of K-562 using 17732-1-AP

    WB result of BCL10 antibody (17732-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BCL10 transfected K-562 cells.

  • IHC staining of human breast cancer using 17732-1-AP

    IHC staining of human breast cancer using 17732-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17732-1-AP (BCL10 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with BCL10 antibody included
  • IHC staining of human breast cancer using 17732-1-AP

    IHC staining of human breast cancer using 17732-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17732-1-AP (BCL10 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with BCL10 antibody included
  • IF Staining of HepG2 using 17732-1-AP

    IF Staining of HepG2 using 17732-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using BCL10 antibody (17732-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

经过测试的应用

Positive WB detected inJurkat cells, K-562 cells
Positive IHC detected inhuman breast cancer tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy BCL10 Ready-To-Use IHC Kit. BCL10 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®555 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17732-1-AP targets BCL10 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen BCL10 fusion protein Ag12170 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name B-cell CLL/lymphoma 10
Synonyms CARD-containing molecule enhancing NF-kappa-B, c E10, Bcl-10, Bcl 10, B-cell lymphoma/leukemia 10
Calculated Molecular Weight 233 aa, 26 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC053617
Gene Symbol BCL10
Gene ID (NCBI) 8915
RRIDAB_2064860
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95999
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The gene encoding BCL10 was identified by its recurrent translocation t(1;14)(p22;q32) in a case of mucosa-associated lymphoid tissue (MALT) lymphoma. BCL10 contains a caspase recruitment domain (CARD), and has been shown to induce apoptosis and to activate NF-kappaB.

实验方案

Product Specific Protocols
WB protocol for BCL10 antibody 17732-1-APDownload protocol
IHC protocol for BCL10 antibody 17732-1-APDownload protocol
IF protocol for BCL10 antibody 17732-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols