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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated BCL10 Polyclonal antibody

BCL10 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-17732

Print datasheet

Synonyms

CARD-containing molecule enhancing NF-kappa-B, c E10, Bcl-10, Bcl 10, B-cell lymphoma/leukemia 10

验证数据展示

  • FC experiment of Jurkat using CL647-17732

    FC experiment of Jurkat using CL647-17732

    1X10^6 Jurkat cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human BCL10 (CL647-17732) (red), or 0.2 ug isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC (Intra) detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy BCL10 Ready-To-Use IHC Kit. BCL10 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 17732-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-17732 targets BCL10 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen BCL10 fusion protein Ag12170 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name B-cell CLL/lymphoma 10
Synonyms CARD-containing molecule enhancing NF-kappa-B, c E10, Bcl-10, Bcl 10, B-cell lymphoma/leukemia 10
Calculated Molecular Weight 233 aa, 26 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC053617
Gene Symbol BCL10
Gene ID (NCBI) 8915
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95999
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The gene encoding BCL10 was identified by its recurrent translocation t(1;14)(p22;q32) in a case of mucosa-associated lymphoid tissue (MALT) lymphoma. BCL10 contains a caspase recruitment domain (CARD), and has been shown to induce apoptosis and to activate NF-kappaB.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 BCL10 antibody CL647-17732Download protocol
Standard Protocols
Click here to view our Standard Protocols