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BCL10 Monoclonal antibody

BCL10 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

2E8G5

Cat no : 66556-1-Ig

Print datasheet

Synonyms

c E10, Bcl-10, Bcl 10, B-cell lymphoma/leukemia 10, B-cell CLL/lymphoma 10

验证数据展示

  • WB analysis using 66556-1-Ig

    WB analysis using 66556-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66556-1-Ig (BCL10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • IHC staining of human tonsillitis using 66556-1-Ig

    IHC staining of human tonsillitis using 66556-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66556-1-Ig (BCL10 antibody) at dilution of 1:700 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with BCL10 antibody included
  • IHC staining of human tonsillitis using 66556-1-Ig

    IHC staining of human tonsillitis using 66556-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66556-1-Ig (BCL10 antibody) at dilution of 1:700 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with BCL10 antibody included
  • IF Staining of HepG2 using 66556-1-Ig

    IF Staining of HepG2 using 66556-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using BCL10 antibody (66556-1-Ig, Clone: 2E8G5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • FC experiment of Jurkat using 66556-1-Ig

    FC experiment of Jurkat using 66556-1-Ig

    1x10^6 Jurkat cells were intracellularly stained with 0.4 ug BCL10 Monoclonal antibody (66556-1-Ig, Clone:2E8G5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inHeLa cells, Raji cells, HepG2 cells, HCT 116 cells, MOLT-4 cells, Jurkat cells, K-562 cells, Ramos cells
Positive IHC detected inhuman tonsillitis tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy BCL10 Ready-To-Use IHC Kit. BCL10 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:350-1:1400
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

66556-1-Ig targets BCL10 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen BCL10 fusion protein Ag12162 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name B-cell CLL/lymphoma 10
Synonyms c E10, Bcl-10, Bcl 10, B-cell lymphoma/leukemia 10, B-cell CLL/lymphoma 10
Calculated Molecular Weight 233 aa, 26 kDa
Observed Molecular Weight 30-32 kDa
GenBank Accession NumberBC053617
Gene Symbol BCL10
Gene ID (NCBI) 8915
RRIDAB_2881917
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO95999
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for BCL10 antibody 66556-1-IgDownload protocol
IHC protocol for BCL10 antibody 66556-1-IgDownload protocol
IF protocol for BCL10 antibody 66556-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Biochem Biophys Res Commun

ZNF436 promotes tumor cell proliferation through transcriptional activation of BCL10 in glioma.

Authors - Yinwu Shang
View Article