BCL10 Monoclonal antibody, PBS Only

Various lysates were subjected to SDS PAGE followed by western blot with 66556-1-Ig (BCL10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66556-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66556-1-Ig (BCL10 antibody) at dilution of 1:700 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66556-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66556-1-Ig (BCL10 antibody) at dilution of 1:700 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66556-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using BCL10 antibody (66556-1-Ig, Clone: 2E8G5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66556-1-PBS in a different storage buffer formulation.

1x10^6 Jurkat cells were intracellularly stained with 0.4 ug BCL10 Monoclonal antibody (66556-1-Ig, Clone:2E8G5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66556-1-PBS in a different storage buffer formulation.