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验证数据展示

  • WB analysis using 84930-3-RR (same clone as 84930-3-PBS)

    WB analysis using 84930-3-RR (same clone as 84930-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84930-3-RR (BCL11A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84930-3-PBS in a different storage buffer formulation.

  • WB analysis using 84930-3-RR (same clone as 84930-3-PBS)

    WB analysis using 84930-3-RR (same clone as 84930-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84930-3-RR (BCL11A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84930-3-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01680-2

    Sandwich ELISA standard curve of MP01680-2

    Sandwich ELISA standard curve of MP01680-2, Human BCL11A Recombinant Matched Antibody Pair - PBS only. 84930-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag2187. 84930-4-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

  • Sample test of MP01680-2

    Sample test of MP01680-2

    The mean BCL11A concentration was determined to be 16.52 ng/mL in U-937 cell extract based on a 2.20 mg/mL extract load.

  • IF Staining of Jurkat using 84930-3-RR (same clone as 84930-3-PBS)

    IF Staining of Jurkat using 84930-3-RR (same clone as 84930-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using BCL11A antibody (84930-3-RR, Clone: 242481F2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84930-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01680-2

    Cytometric bead array standard curve of MP01680-2

    Cytometric bead array standard curve of MP01680-2, BCL11A Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84930-3-PBS. Detection antibody: 84930-4-PBS. Standard: Ag2187. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 84930-3-RR

    Affinity and Kinetic Characterization of 84930-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84930-3-RR against Human BCL11A were performed. The affinity constant is 1.38 nM.

BCL11A Recombinant antibody, PBS Only (Capture)

BCL11A Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84930-3-PBS

产品说明书

CloneNo. 242481F2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

242481F2, B cell lymphoma/leukaemia 11A, B-cell CLL/lymphoma 11A, B-cell lymphoma/leukemia 11A, BCL-11A

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy BCL11A Ready-To-Use IHC Kit. BCL11A primary antibody included.

产品信息

84930-3-PBS targets BCL11A as part of a matched antibody pair:

MP01680-2: 84930-3-PBS capture and 84930-4-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human
免疫原 BCL11A fusion protein Ag2187 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 B-cell CLL/lymphoma 11A (zinc finger protein)
别名 242481F2, B cell lymphoma/leukaemia 11A, B-cell CLL/lymphoma 11A, B-cell lymphoma/leukemia 11A, BCL-11A
计算分子量 835 aa, 91 kDa
观测分子量110-120 kDa
GenBank蛋白编号BC021098
基因名称 BCL11A
Gene ID (NCBI) 53335
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9H165
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C