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验证数据展示

  • WB analysis using 84930-3-RR

    WB analysis using 84930-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84930-3-RR (BCL11A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 84930-3-RR

    WB analysis using 84930-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84930-3-RR (BCL11A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of Jurkat using 84930-3-RR

    IF Staining of Jurkat using 84930-3-RR

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using BCL11A antibody (84930-3-RR, Clone: 242481F2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84930-3-RR

    Affinity and Kinetic Characterization of 84930-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84930-3-RR against Human BCL11A were performed. The affinity constant is 1.38 nM.

BCL11A Recombinant antibody

BCL11A Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84930-3-RR

产品说明书

CloneNo. 242481F2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

242481F2, B cell lymphoma/leukaemia 11A, B-cell CLL/lymphoma 11A, B-cell lymphoma/leukemia 11A, BCL-11A

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, Daudi cells, Raji cells, MOLT-4 cells
Positive IF/ICC detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy BCL11A Ready-To-Use IHC Kit. BCL11A primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84930-3-RR targets BCL11A in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 BCL11A fusion protein Ag2187 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 B-cell CLL/lymphoma 11A (zinc finger protein)
别名 242481F2, B cell lymphoma/leukaemia 11A, B-cell CLL/lymphoma 11A, B-cell lymphoma/leukemia 11A, BCL-11A
计算分子量 835 aa, 91 kDa
观测分子量110-120 kDa
GenBank蛋白编号BC021098
基因名称 BCL11A
Gene ID (NCBI) 53335
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9H165
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for BCL11A antibody 84930-3-RRDownload protocol
IF protocol for BCL11A antibody 84930-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols