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BCL6 Recombinant antibody, PBS Only

BCL6 Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3K6

Cat no : 81754-1-PBS

Print datasheet

Synonyms

BCL-6, BCL 6, B-cell lymphoma 6 protein, B-cell lymphoma 5 protein, B cell lymphoma 6 protein

验证数据展示

  • WB analysis using 81754-1-RR (same clone as 81754-1-PBS)

    WB analysis using 81754-1-RR (same clone as 81754-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81754-1-RR (BCL6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81754-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 81754-1-RR (same clone as 81754-1-PBS)

    IHC staining of human tonsillitis using 81754-1-RR (same clone as 81754-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 81754-1-RR (BCL6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81754-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 81754-1-RR (same clone as 81754-1-PBS)

    IHC staining of human tonsillitis using 81754-1-RR (same clone as 81754-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 81754-1-RR (BCL6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81754-1-PBS in a different storage buffer formulation.

  • IP experiment of Ramos using 81754-1-RR (same clone as 81754-1-PBS)

    IP experiment of Ramos using 81754-1-RR (same clone as 81754-1-PBS)

    IP result of anti-BCL6 (IP:81754-1-RR, 4ug; Detection:81754-1-RR 1:8000) with Ramos cells lysate 1680 ug. This data was developed using the same antibody clone with 81754-1-PBS in a different storage buffer formulation.

  • FC experiment of Ramos using 81754-1-RR (same clone as 81754-1-PBS)

    FC experiment of Ramos using 81754-1-RR (same clone as 81754-1-PBS)

    1X10^6 Ramos cells were intracellularly stained with 0.4 ug Anti-Human BCL6 (81754-1-RR, Clone:3K6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 81754-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81754-1-RR

    Affinity and Kinetic Characterization of 81754-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81754-1-RR against Human BCL6 were performed. The affinity constant is 0.157 nM.

产品信息

81754-1-PBS targets BCL6 in WB, IHC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, FC (Intra), IP, Indirect ELISA Application Description
Tested Reactivity human
Immunogen BCL6 fusion protein Ag15519 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name B-cell CLL/lymphoma 6
Synonyms BCL-6, BCL 6, B-cell lymphoma 6 protein, B-cell lymphoma 5 protein, B cell lymphoma 6 protein
Calculated Molecular Weight 706 aa, 79 kDa
Observed Molecular Weight 79-95 kDa
GenBank Accession NumberBC150184
Gene Symbol BCL6
Gene ID (NCBI) 604
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP41182
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

BCL6, a zinc finger transcription factor, contains an N-terminal BTB/POZ domain and C-terminal zinc finger DNA-binding motifs and represses transcription of a wide range of target proteins and microRNAs. BCL6 protein has been reported as a master regulator of B lymphocyte development and growth, and altered BCL6 protein expression was implicated in pathogenesis of diverse human hematologic malignancies, especially in the diffuse large B cell lymphoma (DLBCL). BCL6 is required for the development of T follicular helper T cells (TFH), a helper T cell subset required for the formation of mature and productive GCs. BCL6 has also been shown to play important regulatory roles in macrophages.