BCL6 Recombinant antibody

BCL6 Recombinant Antibody for WB, IHC, FC (Intra), IP, ELISA
Cat No. 81754-1-RR

产品说明书

CloneNo. 3K6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, FC (Intra), IP, ELISA

BCL-6, BCL 6, B-cell lymphoma 6 protein, B-cell lymphoma 5 protein, B cell lymphoma 6 protein

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive WB detected inRamos cells, A20 cells, Daudi cells
Positive IP detected inRamos cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inRamos cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81754-1-RR targets BCL6 in WB, IHC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, FC (Intra), IP, ELISA Application Description
经测试反应性 human, mouse
免疫原 BCL6 fusion protein Ag15519 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 B-cell CLL/lymphoma 6
别名 BCL-6, BCL 6, B-cell lymphoma 6 protein, B-cell lymphoma 5 protein, B cell lymphoma 6 protein
计算分子量 706 aa, 79 kDa
观测分子量 79-95 kDa
GenBank蛋白编号BC150184
基因名称 BCL6
Gene ID (NCBI) 604
RRIDAB_2935576
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP41182
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BCL6, a zinc finger transcription factor, contains an N-terminal BTB/POZ domain and C-terminal zinc finger DNA-binding motifs and represses transcription of a wide range of target proteins and microRNAs. BCL6 protein has been reported as a master regulator of B lymphocyte development and growth, and altered BCL6 protein expression was implicated in pathogenesis of diverse human hematologic malignancies, especially in the diffuse large B cell lymphoma (DLBCL). BCL6 is required for the development of T follicular helper T cells (TFH), a helper T cell subset required for the formation of mature and productive GCs. BCL6 has also been shown to play important regulatory roles in macrophages.

实验方案

Product Specific Protocols
WB protocol for BCL6 antibody 81754-1-RRDownload protocol
IHC protocol for BCL6 antibody 81754-1-RRDownload protocol
IP protocol for BCL6 antibody 81754-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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