验证数据展示
经过测试的应用
Positive WB detected in | Ramos cells, A20 cells, Daudi cells |
Positive IP detected in | Ramos cells |
Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive FC (Intra) detected in | Ramos cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
81754-1-RR targets BCL6 in WB, IHC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.
经测试应用 | WB, IHC, FC (Intra), IP, ELISA Application Description |
经测试反应性 | human, mouse |
免疫原 | BCL6 fusion protein Ag15519 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | B-cell CLL/lymphoma 6 |
别名 | BCL-6, BCL 6, B-cell lymphoma 6 protein, B-cell lymphoma 5 protein, B cell lymphoma 6 protein |
计算分子量 | 706 aa, 79 kDa |
观测分子量 | 79-95 kDa |
GenBank蛋白编号 | BC150184 |
基因名称 | BCL6 |
Gene ID (NCBI) | 604 |
RRID | AB_2935576 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | P41182 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
BCL6, a zinc finger transcription factor, contains an N-terminal BTB/POZ domain and C-terminal zinc finger DNA-binding motifs and represses transcription of a wide range of target proteins and microRNAs. BCL6 protein has been reported as a master regulator of B lymphocyte development and growth, and altered BCL6 protein expression was implicated in pathogenesis of diverse human hematologic malignancies, especially in the diffuse large B cell lymphoma (DLBCL). BCL6 is required for the development of T follicular helper T cells (TFH), a helper T cell subset required for the formation of mature and productive GCs. BCL6 has also been shown to play important regulatory roles in macrophages.
实验方案
Product Specific Protocols | |
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WB protocol for BCL6 antibody 81754-1-RR | Download protocol |
IHC protocol for BCL6 antibody 81754-1-RR | Download protocol |
IP protocol for BCL6 antibody 81754-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |